Overview

  • Product name

    Anti-ASB8 antibody
  • Description

    Rabbit polyclonal to ASB8
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse, Cow, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human ASB8 (internal sequence). The exact sequence is proprietary.
    Database link: Q9H765

  • Positive control

    • WB: IMR32 whole cell lysate. IHC-P: Human colon carcinoma tissue; U87 xenograft tissue; Rat skin tissue. ICC/IF: HeLa cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.0
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: PBS, 1% BSA, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab227475 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 32 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    May be a substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
  • Tissue specificity

    Highest level of expression in skeletal muscle. Also expressed in heart, brain, placenta, liver, kidney and pancreas.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Contains 4 ANK repeats.
    Contains 1 SOCS box domain.
  • Domain

    The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin-protein ligase complexes.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • Ankyrin repeat and SOCS box containing 8 antibody
    • Ankyrin repeat and SOCS box protein 8 antibody
    • ASB 8 antibody
    • ASB-8 antibody
    • ASB8 antibody
    • ASB8_HUMAN antibody
    • FLJ21255 antibody
    • MGC5540 antibody
    • OTTHUMP00000237000 antibody
    • OTTHUMP00000237001 antibody
    • OTTHUMP00000237007 antibody
    • OTTHUMP00000237009 antibody
    • OTTHUMP00000237010 antibody
    • PP14212 antibody
    see all

Images

  • Anti-ASB8 antibody (ab227475) at 1/1000 dilution + IMR32 (human brain neuroblast cell line) whole cell lysate at 30 µg

    Predicted band size: 32 kDa



    12% SDS-PAGE gel.

  • Paraffin-embedded rat skin tissue stained for ASB8 using ab227475 at 1/500 dilution in immunohistochemical analysis

  • Methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ASB8 (green) using ab227475 at 1/500 dilution in ICC/IF. Nuclear counterstain: Hoechst 33342 (blue).

  • Paraffin-embedded U87 xenograft tissue stained for ASB8 using ab227475 at 1/500 dilution in immunohistochemical analysis

  • Paraffin-embedded human colon carcinoma tissue stained for ASB8 using ab227475 at 1/500 dilution in immunohistochemical analysis

References

ab227475 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab227475.
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