Key features and details
- Rabbit polyclonal to ASB8
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-ASB8 antibody
DescriptionRabbit polyclonal to ASB8
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse, Cow, Rhesus monkey
Recombinant fragment within Human ASB8 (internal sequence). The exact sequence is proprietary.
Database link: Q9H765
- WB: IMR32 whole cell lysate. IHC-P: Human colon carcinoma tissue; U87 xenograft tissue; Rat skin tissue. ICC/IF: HeLa cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227475 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 32 kDa.|
|ICC/IF||1/100 - 1/1000.|
FunctionMay be a substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
Tissue specificityHighest level of expression in skeletal muscle. Also expressed in heart, brain, placenta, liver, kidney and pancreas.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesContains 4 ANK repeats.
Contains 1 SOCS box domain.
DomainThe SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin-protein ligase complexes.
- Information by UniProt
- Ankyrin repeat and SOCS box containing 8 antibody
- Ankyrin repeat and SOCS box protein 8 antibody
- ASB 8 antibody
Anti-ASB8 antibody (ab227475) at 1/1000 dilution + IMR32 (human brain neuroblast cell line) whole cell lysate at 30 µg
Predicted band size: 32 kDa
12% SDS-PAGE gel.
Paraffin-embedded rat skin tissue stained for ASB8 using ab227475 at 1/500 dilution in immunohistochemical analysis
Methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ASB8 (green) using ab227475 at 1/500 dilution in ICC/IF. Nuclear counterstain: Hoechst 33342 (blue).
Paraffin-embedded U87 xenograft tissue stained for ASB8 using ab227475 at 1/500 dilution in immunohistochemical analysis
Paraffin-embedded human colon carcinoma tissue stained for ASB8 using ab227475 at 1/500 dilution in immunohistochemical analysis
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227475 has not yet been referenced specifically in any publications.