Key features and details
- Rabbit polyclonal to ASB9
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ASB9 antibody
DescriptionRabbit polyclonal to ASB9
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant protein fragment, corresponding to a region within amino acids 1-253 of Human ASB9 (NP_001026909).
- HeLaS3, HepG2 and MOLT4 cell lysates for WB; HeLa cells for IF/ICC
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab97918 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 32 kDa.|
|ICC/IF||1/100 - 1/200.|
RelevanceASB9 may be a substrate-recognition component of an SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
- Ankyrin repeat and SOCS box protein 9 antibody
- ASB-9 antibody
Immunohistochemical analysis of paraffin-embedded A549 xenograft, using ab97918 antibody at 1:500 dilution.
All lanes : Anti-ASB9 antibody (ab97918) at 1/1000 dilution
Lane 1 : HeLaS3 whole cell lysate
Lane 2 : HepG2 whole cell lysate
Lane 3 : MOLT4 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 32 kDa
12% SDS PAGE
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab97918 at 1/200 dilution. Lower image merged with DNA probe.
ab97918 has been referenced in 1 publication.
- Benoit G et al. Gonadotropin regulation of ankyrin-repeat and SOCS-box protein 9 (ASB9) in ovarian follicles and identification of binding partners. PLoS One 14:e0212571 (2019). PubMed: 30811458