Anti-ASC1 antibody (ab127537)
Key features and details
- Rabbit polyclonal to ASC1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ASC1 antibody
See all ASC1 primary antibodies -
Description
Rabbit polyclonal to ASC1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 1-272 of Human ASC1 (Q15650).
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Positive control
- A549 and HCT116 whole cell lysates; Human colon carcinoma tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab127537 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 66 kDa.
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 66 kDa. |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Transcription coactivator of nuclear receptors which functions in conjunction with CBP-p300 and SRC-1 and may play an important role in establishing distinct coactivator complexes under different cellular conditions. Plays a pivotal role in the transactivation of NF-kappa-B, SRF and AP1. Acts as a mediator of transrepression between nuclear receptor and either AP1 or NF-kappa-B. Plays a role in androgen receptor transactivation and in testicular function. -
Cellular localization
Nucleus. Cytoplasm. Cytoplasmic under conditions of serum deprivation. - Information by UniProt
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Database links
- Entrez Gene: 533430 Cow
- Entrez Gene: 9325 Human
- Entrez Gene: 56404 Mouse
- Entrez Gene: 315769 Rat
- Omim: 604501 Human
- SwissProt: Q15650 Human
- SwissProt: Q9QXN3 Mouse
- Unigene: 500340 Human
see all -
Alternative names
- Activating signal cointegrator 1 antibody
- ASC 1 antibody
- ASC-1 antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab127537 has been referenced in 5 publications.
- Wu DM et al. Autophagy Induced by Micheliolide Alleviates Acute Irradiation-Induced Intestinal Injury via Inhibition of the NLRP3 Inflammasome. Front Pharmacol 12:773150 (2021). PubMed: 35115927
- Yu Y et al. Bixin Attenuates Experimental Autoimmune Encephalomyelitis by Suppressing TXNIP/NLRP3 Inflammasome Activity and Activating NRF2 Signaling. Front Immunol 11:593368 (2020). PubMed: 33362775
- Qian W et al. Astragaloside IV protects endothelial progenitor cells from the damage of ox-LDL via the LOX-1/NLRP3 inflammasome pathway. Drug Des Devel Ther 13:2579-2589 (2019). PubMed: 31440038
- Zhu J et al. Protective effects of Erigeron breviscapus Hand.- Mazz. (EBHM) extract in retinal neurodegeneration models. Mol Vis 24:315-325 (2018). PubMed: 29769797
- Qi Y et al. Retinal ischemia/reperfusion injury is mediated by Toll-like receptor 4 activation of NLRP3 inflammasomes. Invest Ophthalmol Vis Sci 55:5466-75 (2014). PubMed: 25097240