Key features and details
- Rabbit polyclonal to ASC2/ASCI
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human, Gerbil
- Isotype: IgG
Product nameAnti-ASC2/ASCI antibody
DescriptionRabbit polyclonal to ASC2/ASCI
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human, Gerbil
Recombinant full length protein corresponding to Human ASC2/ASCI.
This product was previously labelled as ASC2
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.05% Sodium azide
Constituent: Whole serum
Concentration information loading...
Our Abpromise guarantee covers the use of ab47092 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/2000. Detects a band of approximately 10 kDa (predicted molecular weight: 10 kDa).|
|IHC-P||Use at an assay dependent concentration.|
FunctionAssociates with apoptosis-associated specklike protein containing a CARD domain (ASC) and modulates its ability to collaborate with pyrin and cryopyrin in NF-kappa-B and pro-caspase-1 activation. Suppresses kinase activity of NF-kappa-B inhibitor kinase (IKK) complex, expression of NF-kappa-B inducible genes and inhibits NF-kappa-B activation by cytokines and LPS.
Tissue specificityPredominantly expressed in monocytes, macrophages and granulocytes.
Sequence similaritiesContains 1 DAPIN domain.
- Information by UniProt
- PAAD domain only protein antibody
- PAAD-only protein 1 antibody
- POP1 antibody
All lanes : Anti-ASC2/ASCI antibody (ab47092) at 1/2000 dilution
Lane 1 : HEK-293 cells transiently transfected with plasmid encoding ASC2/ASCI
Lane 2 : HEK-293 cells transiently transfected with plasmid encoding POP1
Lane 3 : HEK-293 cells transiently transfected with a negative control plasmid
Predicted band size: 10 kDa
Observed band size: 10 kDa
Immunhistochemical analysis of ASC2/ASCI/POP1 expression in mouse spleen using ab47092 at 1:2000. Left and Right images: spleen at low and high magnification, respectively. ASC2/ASCI/POP1 staining is seen in the granulocytes, whereas the lymph and megakaryocytes are negative.
Immunhistochemical analysis of ASC2/ASCI/POP1 expression in mouse using ab47092 at 1:2000. A, prostate tissue from a male mouse prostatitis. B, kidney from a mouse with pyelonephritis. A1 and B1, high magnification of A and B, respectively. ASC2/ASCI/POP1 staining is seen in the granulocytes
ab47092 has been referenced in 3 publications.
- Bai Y et al. Biochanin A attenuates myocardial ischemia/reperfusion injury through the TLR4/NF-?B/NLRP3 signaling pathway. Acta Cir Bras 34:e201901104 (2019). PubMed: 31859817
- Lu J et al. Rapamycin-induced autophagy attenuates hormone-imbalance-induced chronic non-bacterial prostatitis in rats via the inhibition of NLRP3 inflammasome-mediated inflammation. Mol Med Rep N/A:N/A (2018). PubMed: 30483781
- Luo X et al. Baicalein ameliorates TNBS-induced colitis by suppressing TLR4/MyD88 signaling cascade and NLRP3 inflammasome activation in mice. Sci Rep 7:16374 (2017). PubMed: 29180692