Key features and details
- Rabbit polyclonal to ASCC2
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-ASCC2 antibody
See all ASCC2 primary antibodies
DescriptionRabbit polyclonal to ASCC2
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rabbit, Horse, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Snake, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
- HeLa, 293T, Jurkat, mouse TCMK-1 and mouse NIH3T3 whole cell lysates.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab168811 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 86 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionEnhances NF-kappa-B, SRF and AP1 transactivation.
Sequence similaritiesBelongs to the ASCC2 family.
Contains 1 CUE domain.
- Information by UniProt
- 1700011I11Rik antibody
- 2610034L15Rik antibody
- Activating signal cointegrator 1 complex subunit 2 antibody
All lanes : Anti-ASCC2 antibody (ab168811) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK-1 whole cell lysate
Lane 5 : NIH3T3 whole cell lysate
Developed using the ECL technique.
Predicted band size: 86 kDa
Exposure time: 3 minutes
Detection of ASCC2 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab168811 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 10 seconds.
ab168811 has been referenced in 1 publication.
- Chen H et al. Sinomenine Attenuates Acetaminophen-Induced Acute Liver Injury by Decreasing Oxidative Stress and Inflammatory Response via Regulating TGF-ß/Smad Pathway in vitro and in vivo. Drug Des Devel Ther 14:2393-2403 (2020). PubMed: 32606606