Anti-ASCC2 antibody (ab228827)
Key features and details
- Rabbit polyclonal to ASCC2
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ASCC2 antibody
See all ASCC2 primary antibodies -
Description
Rabbit polyclonal to ASCC2 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human ASCC2 (internal sequence). The exact sequence is proprietary.
Database link: Q9H1I8 -
Positive control
- ICC/IF: HeLa cells. IHC-P: Human breast cancer tissue. WB: HeLa and HepG2 whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab228827 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/100 - 1/1000.
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IHC-P |
1/100 - 1/1000.
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WB |
1/1000 - 1/10000. Predicted molecular weight: 86 kDa.
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Notes |
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ICC/IF
1/100 - 1/1000. |
IHC-P
1/100 - 1/1000. |
WB
1/1000 - 1/10000. Predicted molecular weight: 86 kDa. |
Target
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Function
Enhances NF-kappa-B, SRF and AP1 transactivation. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the ASCC2 family.
Contains 1 CUE domain. - Information by UniProt
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Database links
- Entrez Gene: 84164 Human
- Omim: 614216 Human
- SwissProt: Q9H1I8 Human
- Unigene: 517438 Human
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Alternative names
- 1700011I11Rik antibody
- 2610034L15Rik antibody
- Activating signal cointegrator 1 complex subunit 2 antibody
see all
Images
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All lanes : Anti-ASCC2 antibody (ab228827) at 1/5000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 86 kDa7.5% SDS-PAGE gel.
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Methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ASCC2 (green) using ab228827 at 1/500 dilution in ICC/IF.
Blue: Hoechst 33342 staining.
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Paraffin-embedded human breast cancer tissue stained for ASCC2 using ab228827 at 1/500 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab228827 has been referenced in 1 publication.
- Stoneley M et al. Unresolved stalled ribosome complexes restrict cell-cycle progression after genotoxic stress. Mol Cell 82:1557-1572.e7 (2022). PubMed: 35180429