Overview

  • Product name

  • Description

    Rabbit polyclonal to ASCC2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human ASCC2 (internal sequence). The exact sequence is proprietary.
    Database link: Q9H1I8

  • Positive control

    • ICC/IF: HeLa cells. IHC-P: Human breast cancer tissue. WB: HeLa and HepG2 whole cell lysates.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: PBS, 1% BSA, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab228827 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/1000.
IHC-P 1/100 - 1/1000.
WB 1/1000 - 1/10000. Predicted molecular weight: 86 kDa.

Target

  • Function

    Enhances NF-kappa-B, SRF and AP1 transactivation.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the ASCC2 family.
    Contains 1 CUE domain.
  • Information by UniProt
  • Database links

  • Alternative names

    • 1700011I11Rik antibody
    • 2610034L15Rik antibody
    • Activating signal cointegrator 1 complex subunit 2 antibody
    • AI482016 antibody
    • ASC 1 complex subunit p100 antibody
    • ASC-1 complex subunit p100 antibody
    • ASC1P100 antibody
    • ASCC 2 antibody
    • Ascc2 antibody
    • ASCC2_HUMAN antibody
    • AW046480 antibody
    • FLJ21588 antibody
    • OTTMUSP00000005258 antibody
    • p100 antibody
    • RGD1561422 antibody
    • RP23-280J3.3 antibody
    • Trip4 complex subunit p100 antibody
    see all

Images

  • All lanes : Anti-ASCC2 antibody (ab228827) at 1/5000 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 86 kDa



    7.5% SDS-PAGE gel.

  • Methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ASCC2 (green) using ab228827 at 1/500 dilution in ICC/IF.

    Blue: Hoechst 33342 staining.

  • Paraffin-embedded human breast cancer tissue stained for ASCC2 using ab228827 at 1/500 dilution in immunohistochemical analysis.

References

ab228827 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab228827.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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