Key features and details
- Rabbit polyclonal to ASCC2
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ASCC2 antibody
See all ASCC2 primary antibodies
DescriptionRabbit polyclonal to ASCC2
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
Recombinant fragment within Human ASCC2 (internal sequence). The exact sequence is proprietary.
Database link: Q9H1I8
- ICC/IF: HeLa cells. IHC-P: Human breast cancer tissue. WB: HeLa and HepG2 whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab228827 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/1000.|
|IHC-P||1/100 - 1/1000.|
|WB||1/1000 - 1/10000. Predicted molecular weight: 86 kDa.|
FunctionEnhances NF-kappa-B, SRF and AP1 transactivation.
Sequence similaritiesBelongs to the ASCC2 family.
Contains 1 CUE domain.
- Information by UniProt
- 1700011I11Rik antibody
- 2610034L15Rik antibody
- Activating signal cointegrator 1 complex subunit 2 antibody
All lanes : Anti-ASCC2 antibody (ab228827) at 1/5000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 86 kDa
7.5% SDS-PAGE gel.
Methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ASCC2 (green) using ab228827 at 1/500 dilution in ICC/IF.
Blue: Hoechst 33342 staining.
Paraffin-embedded human breast cancer tissue stained for ASCC2 using ab228827 at 1/500 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab228827 has not yet been referenced specifically in any publications.