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Rabbit polyclonal to ASCC3
Predicted to work with:
Synthetic peptide, corresponding to a region within amino acids 2152-2202 of Human ASCC3 (NP_006819.2).
293T, HeLa and Jurkat whole cell lysates.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate Note: pH 7 to 8
Concentration information loading...
Immunogen affinity purified
ab168810 was affinity purified using an epitope specific to ASCC3 immobilized on solid support.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/2500. Predicted molecular weight: 251 kDa.
Use at 2-10 µg/mg of lysate.
Enhances NF-kappa-B, SRF and AP1 transactivation.
Belongs to the helicase family.
Contains 2 helicase ATP-binding domains. Contains 2 helicase C-terminal domains. Contains 3 SEC63 domains.
Information by UniProt
Activating signal cointegrator 1 complex subunit 3 antibody
ASC-1 complex subunit p200 antibody
ASC-1 complex subunit p200-KD subunit antibody
Western blot - Anti-ASCC3 antibody (ab168810)
All lanes : Anti-ASCC3 antibody (ab168810) at 1 µg/ml Lane 1 : 293T whole cell lysate Lane 2 : HeLa whole cell lysate Lane 3 : Jurkat whole cell lysate Lysates/proteins at 50 µg per lane. Developed using the ECL technique. Predicted band size: 251 kDa Exposure time: 3 minutes
Immunoprecipitation - Anti-ASCC3 antibody (ab168810)
Detection of ASCC3 in Immunoprecipitates of 293T whole cell lysate (1 mg for IP, 20% of IP loaded) using ab168810 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 3 minutes.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"