Product nameAnti-ASCC3 antibody
See all ASCC3 primary antibodies
DescriptionRabbit polyclonal to ASCC3
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee
- 293T, HeLa and Jurkat whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
Note: pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab168810 was affinity purified using an epitope specific to ASCC3 immobilized on solid support.
Our Abpromise guarantee covers the use of ab168810 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2500. Predicted molecular weight: 251 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionEnhances NF-kappa-B, SRF and AP1 transactivation.
Sequence similaritiesBelongs to the helicase family.
Contains 2 helicase ATP-binding domains.
Contains 2 helicase C-terminal domains.
Contains 3 SEC63 domains.
- Information by UniProt
- Activating signal cointegrator 1 complex subunit 3 antibody
- ASC-1 complex subunit p200 antibody
- ASC-1 complex subunit p200-KD subunit antibody
All lanes : Anti-ASCC3 antibody (ab168810) at 1 µg/ml
Lane 1 : 293T whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 251 kDa
Exposure time: 3 minutes
Detection of ASCC3 in Immunoprecipitates of 293T whole cell lysate (1 mg for IP, 20% of IP loaded) using ab168810 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 3 minutes.
ab168810 has not yet been referenced specifically in any publications.