Anti-ASCC3 antibody (ab226191)
Key features and details
- Rabbit polyclonal to ASCC3
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-ASCC3 antibody
See all ASCC3 primary antibodies -
Description
Rabbit polyclonal to ASCC3 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Chimpanzee, Gorilla, Orangutan -
Immunogen
Synthetic peptide within Human ASCC3 aa 175-225. The exact sequence is proprietary. (NP_006819.2).
Database link: Q8N3C0 -
Positive control
- WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab226191 was affinity purified using an epitope specific to ASCC3 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab226191 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 251 kDa.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 251 kDa. |
Target
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Function
Enhances NF-kappa-B, SRF and AP1 transactivation. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the helicase family.
Contains 2 helicase ATP-binding domains.
Contains 2 helicase C-terminal domains.
Contains 3 SEC63 domains. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 462898 Chimpanzee
- Entrez Gene: 101134348 Gorilla
- Entrez Gene: 10973 Human
- Entrez Gene: 77987 Mouse
- Entrez Gene: 100436130 Orangutan
- Omim: 614217 Human
- SwissProt: Q8N3C0 Human
- SwissProt: E9PZJ8 Mouse
see all -
Alternative names
- Activating signal cointegrator 1 complex subunit 3 antibody
- ASC-1 complex subunit p200 antibody
- ASC-1 complex subunit p200-KD subunit antibody
see all
Images
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All lanes : Anti-ASCC3 antibody (ab226191) at 0.1 µg/ml
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 251 kDa
Exposure time: 30 seconds
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab226191 has not yet been referenced specifically in any publications.