Ascorbic Acid Assay Kit (ab65346)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Assay time: 30 min
- Sample type: Cell culture supernatant, Other biological fluids, Tissue Extracts, Urine
Overview
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Product name
Ascorbic Acid Assay Kit
See all Ascorbic acid kits -
Detection method
Colorimetric/Fluorometric -
Sample type
Cell culture supernatant, Urine, Other biological fluids, Tissue Extracts -
Assay type
Quantitative -
Range
0.01 nmol/well - 10 nmol/well -
Assay time
0h 30m -
Product overview
Ascorbic Acid Assay Kit ab65346 provides a rapid, simple, and sensitive means of detecting ascorbic acid in various biological samples.
In the ascorbic acid assay protocol, our proprietary catalyst oxidizes ascorbic acid to produce a product that interacts with the ascorbic acid probe, generating color and fluorescence. Ascorbic acid can be easily determined by either colorimetric (spectrophotometry at λ = 570 nm) or fluorometric (Ex/Em = 535/587 nm) methods.
The assay can detect 0.01-10 nmol of ascorbic acid per assay.
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called K661 Ascorbic Acid Colorimetric/Fluorometric Assay Kit. K661-100 is the same size as the 100 test size of ab65346.
Ascorbic Acid (Vitamin C) plays an important role in many biological processes. It is a potent anti-oxidant, anti-inflammatory, anti-viral agent, and an immune stimulant and is present is a wide variety of foods and biological specimens. It is important to be able to monitor ascorbic acid content in these different samples.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Ascorbic Acid Standard 1 vial Assay Buffer II 1 x 25ml Catalyst Reagent 1 x 500µl Developer Solution V 1 vial OxiRed Probe 1 x 0.2ml -
Research areas
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Relevance
Ascorbic Acid (C6H8O6) is an essential nutrient in human diets, and is necessary to maintain connective tissue and bone. The biologically active form of ascorbic acid is vitamic C, a water soluble vitamin which is the L-enantiomer form of ascorbic acid. Humans have lost the ability to synthesize ascorbic acid and must obtain in the diet. Ascorbic acid is an electron donor for enzymes involved in collagen hydroxylation, biosynthesis of carnitine and norepinephrine, tyrosine metabolism and amidatin of peptide hormones. The ability of vitamin C to donate electrons also makes it a potent water-soluble anti-oxidant that readily scavenges free radicals. -
Alternative names
- Vitamin C
Images
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab65346 has been referenced in 6 publications.
- Pesti-Asbóth G et al. Ultrasonication affects the melatonin and auxin levels and the antioxidant system in potato in vitro. Front Plant Sci 13:979141 (2022). PubMed: 36247572
- Choi HI et al. Effects of Acute and Chronic Gamma Irradiation on the Cell Biology and Physiology of Rice Plants. Plants (Basel) 10:N/A (2021). PubMed: 33669039
- Tao B et al. CYB561D2 up-regulation activates STAT3 to induce immunosuppression and aggression in gliomas. J Transl Med 19:338 (2021). PubMed: 34372858
- Chu M et al. Vitamin C Rescues in vitro Embryonic Development by Correcting Impaired Active DNA Demethylation. Front Cell Dev Biol 9:784244 (2021). PubMed: 34869387
- Stsiapura VI et al. S-Nitrosoglutathione formation at gastric pH is augmented by ascorbic acid and by the antioxidant vitamin complex, Resiston. Pharm Biol 56:86-93 (2018). PubMed: 29298528
- Wang C et al. A novel bioactive osteogenesis scaffold delivers ascorbic acid, ß-glycerophosphate, and dexamethasone in vivo to promote bone regeneration. Oncotarget 8:31612-31625 (2017). PubMed: 28404942