Key features and details
- Rabbit polyclonal to Asef2
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Asef2 antibody
See all Asef2 primary antibodies
DescriptionRabbit polyclonal to Asef2
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
- ICC/IF: Human A-431 cells. WB: Human cell line HDLM-2.
Previously labelled as SPATA13.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab122701 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 75 kDa.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.|
FunctionActs as guanine nucleotide exchange factor (GEF) for RHOA, RAC1 and CDC42 GTPases. Regulates cell migration and adhesion assembly and disassembly through a RAC1, PI3K, RHOA and AKT1-dependent mechanism. Increases both RAC1 and CDC42 activity, but decreases the amount of active RHOA. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Involved in tumor angiogenesis and may play a role in intestinal adenoma formation and tumor progression.
Tissue specificityExpressed at high levels in the placenta, spleen and kidney, at moderate levels in lung, small intestine, liver, brain and heart, and at low levels in skeletal muscle. Expression is aberrantly enhanced in most colorectal tumors.
Sequence similaritiesContains 1 DH (DBL-homology) domain.
Contains 1 PH domain.
Contains 1 SH3 domain.
DomainThe C-terminal tail is required for its GEF activity.
Cellular localizationCytoplasm. Cell projection > filopodium. Cell projection > lamellipodium. Cell projection > ruffle membrane. Accumulates in the lamellipodium and ruffle membrane in response to hepatocyte growth factor (HGF) treatment.
- Information by UniProt
- APC-stimulated guanine nucleotide exchange factor 2 antibody
- ARHGEF29 antibody
- Asef2 antibody
ab122701 has been referenced in 1 publication.
- Waseem NH et al. Mutations in SPATA13/ASEF2 cause primary angle closure glaucoma. PLoS Genet 16:e1008721 (2020). PubMed: 32339198