Overview

  • Product name

  • Description

    Rabbit polyclonal to Asef2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat
    Predicted to work with: Human
  • Immunogen

    Recombinant fragment within Human Asef2 (internal sequence). The exact sequence is proprietary.
    Database link: Q96N96

  • Positive control

    • IHC-P: Mouse spleen tissue; rat liver and hind brain tissue.
  • General notes

    Previously labelled as SPATA13.

Properties

Applications

Our Abpromise guarantee covers the use of ab229240 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.

Target

  • Function

    Acts as guanine nucleotide exchange factor (GEF) for RHOA, RAC1 and CDC42 GTPases. Regulates cell migration and adhesion assembly and disassembly through a RAC1, PI3K, RHOA and AKT1-dependent mechanism. Increases both RAC1 and CDC42 activity, but decreases the amount of active RHOA. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Involved in tumor angiogenesis and may play a role in intestinal adenoma formation and tumor progression.
  • Tissue specificity

    Expressed at high levels in the placenta, spleen and kidney, at moderate levels in lung, small intestine, liver, brain and heart, and at low levels in skeletal muscle. Expression is aberrantly enhanced in most colorectal tumors.
  • Sequence similarities

    Contains 1 DH (DBL-homology) domain.
    Contains 1 PH domain.
    Contains 1 SH3 domain.
  • Domain

    The C-terminal tail is required for its GEF activity.
  • Cellular localization

    Cytoplasm. Cell projection > filopodium. Cell projection > lamellipodium. Cell projection > ruffle membrane. Accumulates in the lamellipodium and ruffle membrane in response to hepatocyte growth factor (HGF) treatment.
  • Information by UniProt
  • Database links

  • Alternative names

    • APC-stimulated guanine nucleotide exchange factor 2 antibody
    • ARHGEF29 antibody
    • Asef2 antibody
    • FLJ31208 antibody
    • RP11-307N16.3 antibody
    • Spata13 antibody
    • Spermatogenesis-associated protein 13 antibody
    • SPT13_HUMAN antibody
    see all

Images

  • Paraffin-embedded mouse spleen tissue stained for Asef2 using ab229240 at 1/500 dilution in immunohistochemical analysis.

  • Paraffin-embedded rat liver tissue stained for Asef2 using ab229240 at 1/500 dilution in immunohistochemical analysis.

  • Paraffin-embedded rat hind brain tissue stained for Asef2 using ab229240 at 1/500 dilution in immunohistochemical analysis.

References

ab229240 has not yet been referenced specifically in any publications.

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