Key features and details
- Rabbit polyclonal to Asef2
- Suitable for: IHC-P
- Reacts with: Mouse, Rat
- Isotype: IgG
Product nameAnti-Asef2 antibody
See all Asef2 primary antibodies
DescriptionRabbit polyclonal to Asef2
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Mouse, Rat
Predicted to work with: Human
Recombinant fragment within Human Asef2 (internal sequence). The exact sequence is proprietary.
Database link: Q96N96
- IHC-P: Mouse spleen tissue; rat liver and hind brain tissue.
Previously labelled as SPATA13.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229240 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000.|
FunctionActs as guanine nucleotide exchange factor (GEF) for RHOA, RAC1 and CDC42 GTPases. Regulates cell migration and adhesion assembly and disassembly through a RAC1, PI3K, RHOA and AKT1-dependent mechanism. Increases both RAC1 and CDC42 activity, but decreases the amount of active RHOA. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Involved in tumor angiogenesis and may play a role in intestinal adenoma formation and tumor progression.
Tissue specificityExpressed at high levels in the placenta, spleen and kidney, at moderate levels in lung, small intestine, liver, brain and heart, and at low levels in skeletal muscle. Expression is aberrantly enhanced in most colorectal tumors.
Sequence similaritiesContains 1 DH (DBL-homology) domain.
Contains 1 PH domain.
Contains 1 SH3 domain.
DomainThe C-terminal tail is required for its GEF activity.
Cellular localizationCytoplasm. Cell projection > filopodium. Cell projection > lamellipodium. Cell projection > ruffle membrane. Accumulates in the lamellipodium and ruffle membrane in response to hepatocyte growth factor (HGF) treatment.
- Information by UniProt
- APC-stimulated guanine nucleotide exchange factor 2 antibody
- ARHGEF29 antibody
- Asef2 antibody
Paraffin-embedded mouse spleen tissue stained for Asef2 using ab229240 at 1/500 dilution in immunohistochemical analysis.
Paraffin-embedded rat liver tissue stained for Asef2 using ab229240 at 1/500 dilution in immunohistochemical analysis.
Paraffin-embedded rat hind brain tissue stained for Asef2 using ab229240 at 1/500 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229240 has not yet been referenced specifically in any publications.