Key features and details
- Rabbit polyclonal to ASF1A
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ASF1A antibody
See all ASF1A primary antibodies
DescriptionRabbit polyclonal to ASF1A
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Chicken, Cow
- Recombinant Human ASF1A protein (ab84211) can be used as a positive control in WB. ab24171 gives a positive result in the following whole cell lysates: HeLa (Human epithelial carcinoma cell line), Jurkat (Human T cell lymphoblast-like cell line), A431 (Human epithelial carcinoma cell line) and MCF-7 (Human breast adenocarcinoma cell line).
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab24171 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|WB||1/250. Detects a band of approximately 23, 33 kDa (predicted molecular weight: 23 kDa).|
FunctionHistone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly and with HIRA to promote replication-independent chromatin assembly. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit.
Tissue specificityUbiquitously expressed.
Sequence similaritiesBelongs to the ASF1 family.
modificationsPhosphorylated by TLK1 and TLK2. Highly phosphorylated in S-phase and at lower levels in M-phase.
- Information by UniProt
- Anti silencing function 1A antibody
- Anti-silencing function protein 1 homolog A antibody
- ASF1 anti silencing function 1 homolog A (S. cerevisiae) antibody
All lanes : Anti-ASF1A antibody (ab24171) at 1/250 dilution
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 :
Jurkat whole cell lysate (ab7899)
Lane 3 :
A431 whole cell lysate (ab7909)
Lane 4 : MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 23 kDa
Observed band size: 23,33 kDa why is the actual band size different from the predicted?
Additional bands at: 50 kDa (possible non-specific secondary antibody binding)
ASF1 typically shows up as a band at approximately 33 kDa band on Western blots but has a predicted molecular weight of 23kDa. Occasionally doublet or triplet bands can be observed due to phosphorylation differences.
ICC/IF image of ab24171 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab24171, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
ab24171 has not yet been referenced specifically in any publications.