• Product name

  • Description

    Rabbit polyclonal to ASF1A
  • Host species

  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chicken, Cow
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human ASF1 alpha/beta.

    Read Abcam's proprietary immunogen policy (Peptide available as ab37490.)

  • Positive control

    • Recombinant Human ASF1A protein (ab84211) can be used as a positive control in WB. ab24171 gives a positive result in the following whole cell lysates: HeLa (Human epithelial carcinoma cell line), Jurkat (Human T cell lymphoblast-like cell line), A431 (Human epithelial carcinoma cell line) and MCF-7 (Human breast adenocarcinoma cell line).



Our Abpromise guarantee covers the use of ab24171 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB 1/250. Detects a band of approximately 23, 33 kDa (predicted molecular weight: 23 kDa).


  • Function

    Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly and with HIRA to promote replication-independent chromatin assembly. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit.
  • Tissue specificity

    Ubiquitously expressed.
  • Sequence similarities

    Belongs to the ASF1 family.
  • Post-translational

    Phosphorylated by TLK1 and TLK2. Highly phosphorylated in S-phase and at lower levels in M-phase.
  • Cellular localization

  • Information by UniProt
  • Database links

  • Alternative names

    • Anti silencing function 1A antibody
    • Anti-silencing function protein 1 homolog A antibody
    • ASF1 anti silencing function 1 homolog A (S. cerevisiae) antibody
    • ASF1 anti silencing function 1 homolog A antibody
    • asf1a antibody
    • ASF1A_HUMAN antibody
    • CCG1 interacting factor A antibody
    • CCG1-interacting factor A antibody
    • CGI 98 antibody
    • CIA antibody
    • hAsf1 antibody
    • hAsf1a antibody
    • hCIA antibody
    • Histone chaperone ASF1A antibody
    • HSPC146 antibody
    see all


  • All lanes : Anti-ASF1A antibody (ab24171) at 1/250 dilution

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat whole cell lysate (ab7899)
    Lane 3 : A431 whole cell lysate (ab7909)
    Lane 4 : MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution

    Performed under reducing conditions.

    Predicted band size: 23 kDa
    Observed band size: 23,33 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 50 kDa (possible non-specific secondary antibody binding)

    ASF1 typically shows up as a band at approximately 33 kDa band on Western blots but has a predicted molecular weight of 23kDa.  Occasionally doublet or triplet bands can be observed due to phosphorylation differences.
  • ICC/IF image of ab24171 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab24171, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).


ab24171 has not yet been referenced specifically in any publications.

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