Overview

  • Product name

  • Description

    Rabbit polyclonal to ASF1b
  • Host species

    Rabbit
  • Specificity

    ab70126 detects endogenous levels of total ASF1B protein.
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthesized peptide derived from internal of human ASF1B.

  • Positive control

    • Extracts from K562 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab70126 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 22 kDa.
ELISA 1/5000.

Target

  • Function

    Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly. Does not participate in replication-independent nucleosome deposition which is mediated by ASF1A and HIRA. Required for spermatogenesis.
  • Tissue specificity

    Highly expressed in testis and at lower levels in colon, small intestine and thymus.
  • Sequence similarities

    Belongs to the ASF1 family.
  • Post-translational
    modifications

    Phosphorylated by TLK1 and TLK2.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Anti silencing function 1B antibody
    • Anti silencing function 1B histone chaperone antibody
    • Anti-silencing function protein 1 homolog B antibody
    • ASF1 anti silencing function 1 homolog B (S. cerevisiae) antibody
    • ASF1 anti silencing function 1 homolog B antibody
    • ASF1B antibody
    • ASF1B_HUMAN antibody
    • CCG1 interacting factor A II antibody
    • CCG1-interacting factor A-II antibody
    • CIA-II antibody
    • hAsf1 antibody
    • hAsf1b antibody
    • hCIA-II antibody
    • Histone chaperone ASF1B antibody
    see all

Images

  • All lanes : Anti-ASF1b antibody (ab70126) at 1/500 dilution

    Lane 1 : Extracts from K562 cells
    Lane 2 : Extracts from K562 cells with immunizing peptide at 10 µg

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 22 kDa
    Observed band size: 26 kDa
    why is the actual band size different from the predicted?

References

ab70126 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% Triton X100

Dr. Kirk Mcmanus

Verified customer

Submitted Mar 14 2011

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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