Key features and details
- Rabbit polyclonal to ASF1b
- Suitable for: WB, ELISA
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ASF1b antibody
See all ASF1b primary antibodies
DescriptionRabbit polyclonal to ASF1b
Specificityab70126 detects endogenous levels of total ASF1B protein.
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthesized peptide derived from internal of human ASF1B.
- Extracts from K562 cells
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab70126 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 22 kDa.|
FunctionHistone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly. Does not participate in replication-independent nucleosome deposition which is mediated by ASF1A and HIRA. Required for spermatogenesis.
Tissue specificityHighly expressed in testis and at lower levels in colon, small intestine and thymus.
Sequence similaritiesBelongs to the ASF1 family.
modificationsPhosphorylated by TLK1 and TLK2.
- Information by UniProt
- Anti silencing function 1B antibody
- Anti silencing function 1B histone chaperone antibody
- Anti-silencing function protein 1 homolog B antibody
All lanes : Anti-ASF1b antibody (ab70126) at 1/500 dilution
Lane 1 : Extracts from K562 cells
Lane 2 : Extracts from K562 cells with immunizing peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 22 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?
ab70126 has not yet been referenced specifically in any publications.