Overview

  • Product name

    Anti-ASGR2 antibody [EPR16974]
    See all ASGR2 primary antibodies
  • Description

    Rabbit monoclonal [EPR16974] to ASGR2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human ASGR2 aa 1-100. The exact sequence is proprietary.
    Database link: P07307

  • Positive control

    • Human fetal liver lysate, HepG2 whole cell lysate, Human liver tissue.
  • General notes

     

     

    Previously labelled as Asialoglycoprotein Receptor 2.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR16974
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab197032 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 46 kDa (predicted molecular weight: 35 kDa).
IHC-P 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP 1/70.

Target

  • Function

    Mediates the endocytosis of plasma glycoproteins to which the terminal sialic acid residue on their complex carbohydrate moieties has been removed. The receptor recognizes terminal galactose and N-acetylgalactosamine units. After ligand binding to the receptor, the resulting complex is internalized and transported to a sorting organelle, where receptor and ligand are disassociated. The receptor then returns to the cell membrane surface.
  • Tissue specificity

    Expressed exclusively in hepatic parenchymal cells.
  • Sequence similarities

    Contains 1 C-type lectin domain.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • ASGP R antibody
    • ASGP-R 2 antibody
    • ASGPR 2 antibody
    • ASGPR2 antibody
    • Asgr2 antibody
    • ASGR2_HUMAN antibody
    • Asialoglycoprotein receptor 2 antibody
    • C type lectin domain family 4 member H2 antibody
    • C-type lectin domain family 4 member H2 antibody
    • CLEC4H2 antibody
    • HBxAg-binding protein antibody
    • HBXBP antibody
    • Hepatic lectin 2 antibody
    • Hepatic lectin H2 antibody
    • Hepatic lectin R2/3 antibody
    • HL-2 antibody
    • Hs.1259 antibody
    • L H2 antibody
    • LH2 antibody
    • MGC108546 antibody
    • MHL 2 antibody
    • RHL2 antibody
    see all

Images

  • All lanes : Anti-ASGR2 antibody [EPR16974] (ab197032) at 1/1000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : MCF-7 (Human breast adenocarcinoma cell line) whole cell lysate
    Lane 3 : K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysate
    Lane 4 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate
    Lane 5 : HeLa (Human epithetial cells from cervix adenocarcinoma) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 35 kDa
    Observed band size: 46 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking and diluting buffer was 5% NFDM/TBST. 

    The observed MW is consistent with that described in the literature (PMID:3040719). 

  • Anti-ASGR2 antibody [EPR16974] (ab197032) at 1/2000 dilution + HepG2 (Human liver hepatocellular carcinoma) whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 35 kDa
    Observed band size: 46 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking and diluting buffer was 5% NFDM/TBST.
    The observed MW is consistent with that described in the literature (PMID:3040719).

  • Immunohistochemical analysis of paraffin-embedded human liver tissue labeling ASGR2 with ab197032 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500. Cell membrane staining on Human liver tissue is observed; Uniprot suggests Cell membrane as subcellular location. Counter stained with Hematoxylin.

    Negative control: Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded Human tonsil tissue (negative control tissue) using  ab197032 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500. No staining on Human tonsil tissue is observed. Counter stained with Hematoxylin.
    Negative control: Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • ASGR2 was immunoprecipitated from Human fetal liver with ab197032 at 1/70 dilution. Western blot was performed from the immunoprecipitate using ab197032 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1: Human fetal liver. Lane 2: Rabbit monoclonal IgG (ab172730) instead of ab197032 in Human fetal liver.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

     

References

ab197032 has not yet been referenced specifically in any publications.

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