Recombinant
RabMAb

Recombinant Anti-ASGR2 antibody [EPR16975] - BSA and Azide free (ab216521)

Overview

  • Product name

    Anti-ASGR2 antibody [EPR16975] - BSA and Azide free
    See all ASGR2 primary antibodies
  • Description

    Rabbit monoclonal [EPR16975] to ASGR2 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    within Human ASGR2 aa 200-300. The exact sequence is proprietary.
    Database link: P07307

  • Positive control

    • WB: Human fetal liver lysate. IHC-P: Human liver tissue. IP: Human fetal liver whole cell lysate.
  • General notes

    ab216521 is the carrier-free version of ab200196 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab216521 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Previously labelled as Asialoglycoprotein Receptor 2.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR16975
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab216521 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 46 kDa (predicted molecular weight: 35 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP Use at an assay dependent concentration.

Target

  • Function

    Mediates the endocytosis of plasma glycoproteins to which the terminal sialic acid residue on their complex carbohydrate moieties has been removed. The receptor recognizes terminal galactose and N-acetylgalactosamine units. After ligand binding to the receptor, the resulting complex is internalized and transported to a sorting organelle, where receptor and ligand are disassociated. The receptor then returns to the cell membrane surface.
  • Tissue specificity

    Expressed exclusively in hepatic parenchymal cells.
  • Sequence similarities

    Contains 1 C-type lectin domain.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • ASGP R antibody
    • ASGP-R 2 antibody
    • ASGPR 2 antibody
    • ASGPR2 antibody
    • Asgr2 antibody
    • ASGR2_HUMAN antibody
    • Asialoglycoprotein receptor 2 antibody
    • C type lectin domain family 4 member H2 antibody
    • C-type lectin domain family 4 member H2 antibody
    • CLEC4H2 antibody
    • HBxAg-binding protein antibody
    • HBXBP antibody
    • Hepatic lectin 2 antibody
    • Hepatic lectin H2 antibody
    • Hepatic lectin R2/3 antibody
    • HL-2 antibody
    • Hs.1259 antibody
    • L H2 antibody
    • LH2 antibody
    • MGC108546 antibody
    • MHL 2 antibody
    • RHL2 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling ASGR2  with ab200196 at 1/600 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.

    No staining on Human skeletal muscle tissue is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab200196).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • ASGR2 was immunoprecipitated from 1mg of Human fetal liver whole cell lysate with ab200196 at 1/30 dilution.

    Western blot was performed from the immunoprecipitate using ab200196 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: Human fetal liver whole cell lysate 10 µg (Input).

    Lane 2: ab200196 IP in Human fetal liver whole cell lysate. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab200196 in Human fetal liver whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab200196).

  • This IHC data was generated using the same anti-Asiaglycoprotein Receptor 2 antibody clone, EPR16975, in a different buffer formulation (cat# ab200196).

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ASGR2 with ab200196 at 1/600 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.

    Cytoplasm and cell membrane staining on Human liver tissue is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

     

References

ab216521 has not yet been referenced specifically in any publications.

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