Key features and details
- Rabbit polyclonal to ASH2L
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ASH2L antibody
See all ASH2L primary antibodies
DescriptionRabbit polyclonal to ASH2L
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Recombinant full length protein corresponding to Human ASH2L.
Database link: Q9UBL3
- WB: HeLa nuclear extract; Ectopically expressed DDDDK-tagged ASH2L.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Concentration information loading...
Our Abpromise guarantee covers the use of ab230385 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 69 kDa.|
FunctionComponent of the Set1/Ash2 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3, but not if the neighboring 'Lys-9' residue is already methylated. As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. May function as a transcriptional regulator. May play a role in hematopoiesis.
Tissue specificityUbiquitously expressed. Predominantly expressed in adult heart and testis and fetal lung and liver, with barely detectable expression in adult lung, liver, kidney, prostate, and peripheral leukocytes.
Sequence similaritiesContains 1 B30.2/SPRY domain.
- Information by UniProt
- ASH 2 antibody
- Ash2 (absent small or homeotic) like antibody
- Ash2 (absent, small, or homeotic) like (Drosophila) antibody
All lanes : Anti-ASH2L antibody (ab230385) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) nuclear extract
Lane 2 : Ectopically expressed DDDDK-tagged ASH2L
Predicted band size: 69 kDa
The lower panel shows the same western blot with an anti-DDDDK antibody.
Anti-ASH2L antibody (ab230385) at 1/1000 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) nuclear extract at 20 µg
Predicted band size: 69 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230385 has not yet been referenced specifically in any publications.