Product nameAnti-ASH2L antibody [EPR13106]
See all ASH2L primary antibodies
DescriptionRabbit monoclonal [EPR13106] to ASH2L
Tested applicationsSuitable for: WB, ICC/IF, Flow Cyt, IPmore details
Species reactivityReacts with: Rat, Human
Synthetic peptide within Human ASH2L aa 50-150. The exact sequence is proprietary.
Database link: Q9UBL3
- HeLa cells, 293T cell line lysate, K562 cell line lysate, C6 cell line lysate, PC12 cell line lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab181117 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 69 kDa).|
|ICC/IF||1/50 - 1/100.|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionComponent of the Set1/Ash2 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3, but not if the neighboring 'Lys-9' residue is already methylated. As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. May function as a transcriptional regulator. May play a role in hematopoiesis.
Tissue specificityUbiquitously expressed. Predominantly expressed in adult heart and testis and fetal lung and liver, with barely detectable expression in adult lung, liver, kidney, prostate, and peripheral leukocytes.
Sequence similaritiesContains 1 B30.2/SPRY domain.
- Information by UniProt
- ASH 2 antibody
- Ash2 (absent small or homeotic) like antibody
- Ash2 (absent, small, or homeotic) like (Drosophila) antibody
All lanes : Anti-ASH2L antibody [EPR13106] (ab181117) at 1/10000 dilution
Lane 1 : 293T cell line lysate
Lane 2 : K562 cell line lysate
Lane 3 : C6 cell line lysate
Lane 4 : PC12 cell line lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution
Predicted band size: 69 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
Flow cytometric analysis of 2% paraformaldehyde fixed HeLa cells labeling ASH2L with ab181117 at 1/20 followed by Goat anti rabbit IgG (FITC) at 1/150 (pink). Rabbit monoclonal IgG was used as Isotype control (green).
Immunofluorescent analysis of acetone fixed HeLa cells labeling ASH2L with ab181117 at 1/100 (green). Staining with Dapi is shown in blue.
Immunoprecipitation analysis of 293T cells labeling ASH2L using ab181117 at 1/60 dilution. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/2000 was used as secondary antibody.
ab181117 has not yet been referenced specifically in any publications.