Recombinant Anti-ASH2L antibody [EPR13106] (ab181117)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13106] to ASH2L
- Suitable for: Flow Cyt (Intra), WB, ICC/IF, IP
- Reacts with: Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-ASH2L antibody [EPR13106]
See all ASH2L primary antibodies -
Description
Rabbit monoclonal [EPR13106] to ASH2L -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, ICC/IF, IPmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa cells, 293T cell line lysate, K562 cell line lysate, C6 cell line lysate, PC12 cell line lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR13106 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181117 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/20.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/1000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 69 kDa).
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ICC/IF |
1/50 - 1/100.
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IP |
1/60.
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Notes |
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Flow Cyt (Intra)
1/20. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 69 kDa). |
ICC/IF
1/50 - 1/100. |
IP
1/60. |
Target
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Function
Component of the Set1/Ash2 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3, but not if the neighboring 'Lys-9' residue is already methylated. As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. May function as a transcriptional regulator. May play a role in hematopoiesis. -
Tissue specificity
Ubiquitously expressed. Predominantly expressed in adult heart and testis and fetal lung and liver, with barely detectable expression in adult lung, liver, kidney, prostate, and peripheral leukocytes. -
Sequence similarities
Contains 1 B30.2/SPRY domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 9070 Human
- Entrez Gene: 290829 Rat
- Omim: 604782 Human
- SwissProt: Q9UBL3 Human
- Unigene: 521530 Human
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Alternative names
- ASH 2 antibody
- Ash2 (absent small or homeotic) like antibody
- Ash2 (absent, small, or homeotic) like (Drosophila) antibody
see all
Images
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All lanes : Anti-ASH2L antibody [EPR13106] (ab181117) at 1/10000 dilution
Lane 1 : 293T cell lysate
Lane 2 : K562 cell lysate
Lane 3 : C6 cell lysate
Lane 4 : PC-12 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 69 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted? -
Intracellular flow cytometric analysis of2% paraformaldehyde fixed HeLa cells labeling ASH2L with ab181117 at 1/20 followed byGoat anti rabbit IgG (FITC) at 1/150 (pink).Rabbit monoclonal IgG was used as Isotype control (green).
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Immunofluorescent analysis of acetone fixed HeLa cells labeling ASH2L with ab181117 at 1/100 (green). Staining with Dapi is shown in blue.
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Immunoprecipitation analysis of 293T cells labeling ASH2L using ab181117 at 1/60 dilution. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/2000 was used as secondary antibody.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab181117 has been referenced in 1 publication.
- Wu YJ et al. ASH2L-Promoted HOXC8 Gene Expression Plays a Role in Mixed Lineage Leukemia-Rearranged Acute Leukemia. Onco Targets Ther 13:381-387 (2020). PubMed: 32021283