Product nameAnti-ASH2L antibody [EPR13107(B)] - Nuclear Marker
See all ASH2L primary antibodies
DescriptionRabbit monoclonal [EPR13107(B)] to ASH2L - Nuclear Marker
Tested applicationsSuitable for: ICC/IF, Flow Cyt, IHC-P, WBmore details
Unsuitable for: IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human ASH2L aa 600 to the C-terminus (C terminal) (Cysteine residue). The exact sequence is proprietary.
Database link: Q9UBL3
- WB: HeLa, 293T, Jurkat and K562 cell lysates. IHC-P: Human colon and testis tissues. ICC/IF: HeLa cells. Flow Cyt: HeLa cells.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab176334 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/250.|
|IHC-P||1/50 - 1/100.|
|WB||1/10000 - 1/50000. Predicted molecular weight: 69 kDa.|
FunctionComponent of the Set1/Ash2 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3, but not if the neighboring 'Lys-9' residue is already methylated. As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. May function as a transcriptional regulator. May play a role in hematopoiesis.
Tissue specificityUbiquitously expressed. Predominantly expressed in adult heart and testis and fetal lung and liver, with barely detectable expression in adult lung, liver, kidney, prostate, and peripheral leukocytes.
Sequence similaritiesContains 1 B30.2/SPRY domain.
- Information by UniProt
- ASH 2 antibody
- Ash2 (absent small or homeotic) like antibody
- Ash2 (absent, small, or homeotic) like (Drosophila) antibody
Immunofluorescent analysis of HeLa labeling ASH2Lwith ab176334 at 1/100 dilution.
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeing ASH2L with ab176334 at 1/50 dilution.
Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling ASH2L (red) with ab176334 at a 1/1200 dilution. Cells were fixed with 80% methanol and permeabilized with 0.1% Tween-20. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.
All lanes : Anti-ASH2L antibody [EPR13107(B)] - Nuclear Marker (ab176334) at 1/10000 dilution
Lane 1 : HeLa cell lysates
Lane 2 : 293T cell lysates
Lane 3 : Jurkat cell lysates
Lane 4 : K562 cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 69 kDa
Immunohistochemical analysis of paraffin-embedded Human testis tissue labeing ASH2L with ab176334 at 1/50 dilution.
ab176334 has not yet been referenced specifically in any publications.