Anti-Asialoglycoprotein Receptor 1/HL-1 antibody (ab127896)
Key features and details
- Rabbit polyclonal to Asialoglycoprotein Receptor 1/HL-1
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Asialoglycoprotein Receptor 1/HL-1 antibody
See all Asialoglycoprotein Receptor 1/HL-1 primary antibodies -
Description
Rabbit polyclonal to Asialoglycoprotein Receptor 1/HL-1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to Human Asialoglycoprotein Receptor 1/HL-1 aa 25-241.
Database link: P07306 -
Positive control
- WB: HepG2 and Mouse NIH/3T3 cell lysates. Mouse liver tissue extract. ICC/IF: HepG2 cells.
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General notes
Previously labelled as Asialoglycoprotein Receptor 1.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab127896 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/100 - 1/1000.
|
|
| WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 33 kDa.
|
| Notes |
|---|
|
ICC/IF
1/100 - 1/1000. |
|
WB
1/500 - 1/3000. Predicted molecular weight: 33 kDa. |
Target
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Function
Mediates the endocytosis of plasma glycoproteins to which the terminal sialic acid residue on their complex carbohydrate moieties has been removed. The receptor recognizes terminal galactose and N-acetylgalactosamine units. After ligand binding to the receptor, the resulting complex is internalized and transported to a sorting organelle, where receptor and ligand are disassociated. The receptor then returns to the cell membrane surface. -
Tissue specificity
Expressed exclusively in hepatic parenchymal cells. -
Sequence similarities
Contains 1 C-type lectin domain. -
Post-translational
modificationsPhosphorylated on a cytoplasmic Ser residue. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 432 Human
- Entrez Gene: 11889 Mouse
- Omim: 108360 Human
- SwissProt: P07306 Human
- SwissProt: P34927 Mouse
- Unigene: 12056 Human
- Unigene: 6559 Mouse
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Alternative names
- ASGP-R 1 antibody
- ASGPR 1 antibody
- ASGPR antibody
see all
Images
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Western blot - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody (ab127896)Anti-Asialoglycoprotein Receptor 1/HL-1 antibody (ab127896) at 1/3000 dilution + Mouse liver tissue extract at 50 µg
Predicted band size: 33 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody (ab127896)HepG2 cells stained for ASGPR1 (green) using ab127896 at 1/500 dilution in ICC/IF. Counterstaining using Hoechst 33342 staining (blue).
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Western blot - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody (ab127896)Anti-Asialoglycoprotein Receptor 1/HL-1 antibody (ab127896) at 1/3000 dilution + HepG2 whole cell lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 33 kDa
12% SDS Page -
Western blot - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody (ab127896)Anti-Asialoglycoprotein Receptor 1/HL-1 antibody (ab127896) at 1/1000 dilution + Mouse NIH/3T3 whole cell lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 33 kDa
12% SDS Page
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab127896 has not yet been referenced specifically in any publications.