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Signal Transduction Protein Trafficking Organelle Proteins
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Anti-Asialoglycoprotein Receptor 1/HL-1 antibody (ab127896)

  • Datasheet
  • SDS
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Western blot - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody (ab127896)
  • Immunocytochemistry/ Immunofluorescence - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody (ab127896)
  • Western blot - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody (ab127896)
  • Western blot - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody (ab127896)

Key features and details

  • Rabbit polyclonal to Asialoglycoprotein Receptor 1/HL-1
  • Suitable for: ICC/IF, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

You may also be interested in

Protein
Recombinant Human Asialoglycoprotein Receptor 1/HL-1 (ab151893)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-Asialoglycoprotein Receptor 1/HL-1 antibody
    See all Asialoglycoprotein Receptor 1/HL-1 primary antibodies
  • Description

    Rabbit polyclonal to Asialoglycoprotein Receptor 1/HL-1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to Human Asialoglycoprotein Receptor 1/HL-1 aa 25-241.
    Database link: P07306

  • Positive control

    • WB: HepG2 and Mouse NIH/3T3 cell lysates. Mouse liver tissue extract. ICC/IF: HepG2 cells.
  • General notes

    Previously labelled as Asialoglycoprotein Receptor 1. 

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Trafficking
    • Organelle Proteins

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • NIH/3T3 whole cell lysate (ab7179)
  • Recombinant Protein

    • Recombinant Human Asialoglycoprotein Receptor 1/HL-1 (ab151893)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab127896 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/100 - 1/1000.
WB (1)
1/500 - 1/3000. Predicted molecular weight: 33 kDa.
Notes
ICC/IF
1/100 - 1/1000.
WB
1/500 - 1/3000. Predicted molecular weight: 33 kDa.

Target

  • Function

    Mediates the endocytosis of plasma glycoproteins to which the terminal sialic acid residue on their complex carbohydrate moieties has been removed. The receptor recognizes terminal galactose and N-acetylgalactosamine units. After ligand binding to the receptor, the resulting complex is internalized and transported to a sorting organelle, where receptor and ligand are disassociated. The receptor then returns to the cell membrane surface.
  • Tissue specificity

    Expressed exclusively in hepatic parenchymal cells.
  • Sequence similarities

    Contains 1 C-type lectin domain.
  • Post-translational
    modifications

    Phosphorylated on a cytoplasmic Ser residue.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P07306 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 432 Human
    • Entrez Gene: 11889 Mouse
    • Omim: 108360 Human
    • SwissProt: P07306 Human
    • SwissProt: P34927 Mouse
    • Unigene: 12056 Human
    • Unigene: 6559 Mouse
    • Alternative names

      • ASGP-R 1 antibody
      • ASGPR 1 antibody
      • ASGPR antibody
      • ASGPR1 antibody
      • Asgr1 antibody
      • ASGR1_HUMAN antibody
      • Asialoglycoprotein receptor 1 antibody
      • C type lectin domain family 4 member H1 antibody
      • C-type lectin domain family 4 member H1 antibody
      • CLEC4H1 antibody
      • Hepatic lectin H1 antibody
      • HL-1 antibody
      see all

    Images

    • Western blot - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody (ab127896)
      Western blot - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody (ab127896)
      Anti-Asialoglycoprotein Receptor 1/HL-1 antibody (ab127896) at 1/3000 dilution + Mouse liver tissue extract at 50 µg

      Predicted band size: 33 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody (ab127896)
      Immunocytochemistry/ Immunofluorescence - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody (ab127896)

      HepG2 cells stained for ASGPR1 (green) using ab127896 at 1/500 dilution in ICC/IF. Counterstaining using Hoechst 33342 staining (blue).

    • Western blot - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody (ab127896)
      Western blot - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody (ab127896)
      Anti-Asialoglycoprotein Receptor 1/HL-1 antibody (ab127896) at 1/3000 dilution + HepG2 whole cell lysate at 30 µg

      Developed using the ECL technique.

      Predicted band size: 33 kDa



      12% SDS Page
    • Western blot - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody (ab127896)
      Western blot - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody (ab127896)
      Anti-Asialoglycoprotein Receptor 1/HL-1 antibody (ab127896) at 1/1000 dilution + Mouse NIH/3T3 whole cell lysate at 30 µg

      Developed using the ECL technique.

      Predicted band size: 33 kDa



      12% SDS Page

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab127896? Please let us know so that we can cite the reference in this datasheet.

    ab127896 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
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    Western blot abreview for Anti-Asialoglycoprotein Receptor 1/HL-1 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Mice hepatocytes)
    Gel Running Conditions
    Reduced Denaturing (4-15% SDS-PAGE)
    Loading amount
    35 µg
    Specification
    Mice hepatocytes
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Dr. Armen Petrosyan

    Verified customer

    Submitted Mar 16 2021

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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