Anti-Asialoglycoprotein Receptor 1/HL-1 antibody (ab231548)

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Overview

  • Product name

    Anti-Asialoglycoprotein Receptor 1/HL-1 antibody
    See all Asialoglycoprotein Receptor 1/HL-1 primary antibodies

  • Description

    Rabbit polyclonal to Asialoglycoprotein Receptor 1/HL-1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human, Pig
    Predicted to work with: Orangutan

  • Immunogen

    Recombinant fragment (His-tag) corresponding to Human Asialoglycoprotein Receptor 1/HL-1 aa 77-289. N-terminal tag. Expressed in E.coli.
    Sequence:

    FSNFTASTEAQVKGLSTQGGNVGRKMKSLESQLEKQQKDLSEDHSSLLLH VKQFVSDLRSLSCQMAALQGNGSERTCCPVNWVEHERSCYWFSRSGKAWA DADNYCRLEDAHLVVVTSWEEQKFVQHHIGPVNTWMGLHDQNGPWKWVDG TDYETGFKNWRPEQPDDWYGHGLGGGEDCAHFTDDGRWNDDVCQRPYRWV CETELDKASQEPP


    Database link: P07306

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • IHC-P: Human liver tissue. WB: Pig liver lysate; Recombinant human Asialoglycoprotein Receptor 1/HL-1 protein.

  • General notes

    Previously labelled as Asialoglycoprotein Receptor 1. 

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    ab231548 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab231548 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 33 kDa.
IHC-P Use a concentration of 5 - 20 µg/ml.

Target

  • Function

    Mediates the endocytosis of plasma glycoproteins to which the terminal sialic acid residue on their complex carbohydrate moieties has been removed. The receptor recognizes terminal galactose and N-acetylgalactosamine units. After ligand binding to the receptor, the resulting complex is internalized and transported to a sorting organelle, where receptor and ligand are disassociated. The receptor then returns to the cell membrane surface.

  • Tissue specificity

    Expressed exclusively in hepatic parenchymal cells.

  • Sequence similarities

    Contains 1 C-type lectin domain.

  • Post-translational
    modifications

    Phosphorylated on a cytoplasmic Ser residue.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • ASGP-R 1 antibody
      • ASGPR 1 antibody
      • ASGPR antibody
      • ASGPR1 antibody
      • Asgr1 antibody
      • ASGR1_HUMAN antibody
      • Asialoglycoprotein receptor 1 antibody
      • C type lectin domain family 4 member H1 antibody
      • C-type lectin domain family 4 member H1 antibody
      • CLEC4H1 antibody
      • Hepatic lectin H1 antibody
      • HL-1 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody (ab231548)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody (ab231548)

      Paraffin-embedded human liver tissue stained for Asialoglycoprotein Receptor 1/HL-1 using ab231548 at 20 µg/ml in immunohistochemical analysis. DAB staining.

    • Western blot - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody (ab231548)
      Western blot - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody (ab231548)
      Anti-Asialoglycoprotein Receptor 1/HL-1 antibody (ab231548) at 3 µg/ml + Pig liver lysate

      Secondary
      HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution

      Predicted band size: 33 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody (ab231548)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody (ab231548)

      Paraffin-embedded human liver tissue stained for Asialoglycoprotein Receptor 1/HL-1 using ab231548 at 20 µg/ml in immunohistochemical analysis. DAB staining.

    • Western blot - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody (ab231548)
      Western blot - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody (ab231548)
      Anti-Asialoglycoprotein Receptor 1/HL-1 antibody (ab231548) at 3 µg/ml + Recombinant human Asialoglycoprotein Receptor 1/HL-1 protein

      Predicted band size: 33 kDa

    References

    ab231548 has not yet been referenced specifically in any publications.

    Publishing research using ab231548? Please let us know so that we can cite the reference in this datasheet.

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