Western blot abreview for Anti-Asialoglycoprotein Receptor 1/HL-1 antibody

Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HepG2 cells)
Gel Running Conditions
Reduced Denaturing (12% SDS-PAGE)
Loading amount
26 µg
Specification
HepG2 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA in PBS
Dilution
1/2000

Secondary antibody

Dilution
1/10000
Name
Non-Abcam antibody was used: Donkey-anti-Rb
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL+
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: between 55 and 40 kDa Non-specific: around 38 kDa kDa

Dr. Armen Petrosyan

Verified customer

Submitted Oct 21 2016

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