Immunoprecipitation abreview for Anti-Asialoglycoprotein Receptor 1 / HL-1 antibody

Excellent
Abreviews
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (HepG2 cells)
Total protein in input
400 µg
Treatment
Control and treated with 35 mM ethanol
Immuno-precipitation step
Other - Dynabeads M-270 Epoxy beads (Pierce)
Specification
HepG2 cells

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: IP lysis buffer (Pierce - cat # 87788)
Dilution
1/50

Western Blot antibody

Western Blot antibody
None used

Additional data

Additional Notes
SNA lectin W-B of ASGP-R-IP from the plasma membrane fractions isolated from VA-13 cells: control, EtOH-treated, and recovered from EtOH in absence or presence of giantin siRNAs. The input was normalized to the E-cadherin
PubMed ID
30453527: View

Dr. Armen Petrosyan

Verified customer

Submitted Feb 14 2019

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