RecombinantRabMAb

Recombinant Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22639-68] - BSA and Azide free (ab256352)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22639-68] - BSA and Azide free (ab256352)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22639-68] - BSA and Azide free (ab256352)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22639-68] - BSA and Azide free (ab256352)
  • Immunocytochemistry/ Immunofluorescence - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22639-68] - BSA and Azide free (ab256352)
  • Immunoprecipitation - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22639-68] - BSA and Azide free (ab256352)
  • Immunoprecipitation - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22639-68] - BSA and Azide free (ab256352)
  • Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22639-68] - BSA and Azide free (ab256352)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22639-68] to Asialoglycoprotein Receptor 1/HL-1 - BSA and Azide free
  • Suitable for: WB, ICC/IF, IHC-P, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22639-68] - BSA and Azide free
    See all Asialoglycoprotein Receptor 1/HL-1 primary antibodies

  • Description

    Rabbit monoclonal [EPR22639-68] to Asialoglycoprotein Receptor 1/HL-1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, IHC-P, IPmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human Asialoglycoprotein Receptor 1/HL-1 aa 50-150. The exact sequence is proprietary.
    Database link: P07306

  • Positive control

    • WB: HepG2 and Huh7 cell lysates whole cell lysate. Human liver tissue lysate. IP: HepG2 whole cell lysates IHC-P Human liver and liver cancer tissue ICC/IF: HepG2 cells.

  • General notes

    Ab256352 is the carrier-free version of ab254261. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab256352 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR22639-68

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab256352 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 33 kDa.
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for Flow Cyt.

    • Target

    • Function

      Mediates the endocytosis of plasma glycoproteins to which the terminal sialic acid residue on their complex carbohydrate moieties has been removed. The receptor recognizes terminal galactose and N-acetylgalactosamine units. After ligand binding to the receptor, the resulting complex is internalized and transported to a sorting organelle, where receptor and ligand are disassociated. The receptor then returns to the cell membrane surface.

    • Tissue specificity

      Expressed exclusively in hepatic parenchymal cells.

    • Sequence similarities

      Contains 1 C-type lectin domain.

    • Post-translational
      modifications

      Phosphorylated on a cytoplasmic Ser residue.

    • Cellular localization

      Membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • ASGP-R 1 antibody
        • ASGPR 1 antibody
        • ASGPR antibody
        • ASGPR1 antibody
        • Asgr1 antibody
        • ASGR1_HUMAN antibody
        • Asialoglycoprotein receptor 1 antibody
        • C type lectin domain family 4 member H1 antibody
        • C-type lectin domain family 4 member H1 antibody
        • CLEC4H1 antibody
        • Hepatic lectin H1 antibody
        • HL-1 antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22639-68] - BSA and Azide free (ab256352)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22639-68] - BSA and Azide free (ab256352)

        Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Asialoglycoprotein Receptor 1/HL-1 with ab254261 at 1/2000 dilution (0.25 μg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in human liver (PMID:23979840,26422581,27241665). Counterstained with hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

        Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254261).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22639-68] - BSA and Azide free (ab256352)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22639-68] - BSA and Azide free (ab256352)

        Immunohistochemical analysis of paraffin-embedded human liver cancer tissue labeling Asialoglycoprotein Receptor 1/HL-1 with ab254261 at 1/2000 dilution (0.25 μg/ml)  followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in human liver cancer (PMID:23979840,26422581,27241665). Counterstained with hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

        Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254261).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22639-68] - BSA and Azide free (ab256352)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22639-68] - BSA and Azide free (ab256352)

        Negative control: No staining in human colon (PMID:23979840).

        Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Asialoglycoprotein Receptor 1/HL-1 with ab254261 at 1/2000 dilution (0.25 μg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Counterstained with hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

        Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254261).

      • Immunocytochemistry/ Immunofluorescence - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22639-68] - BSA and Azide free (ab256352)
        Immunocytochemistry/ Immunofluorescence - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22639-68] - BSA and Azide free (ab256352)

        Immunofluorescent analysis of 100% Methanol-fixed HepG2 (human hepatocellular carcinoma epithelial cell) cells labeling Asialoglycoprotein Receptor 1/HL-1 with at 1/50 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in HepG2 cell line is observed. Methanol fixation is preferred.

        ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)  was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

        Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254261).

      • Immunoprecipitation - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22639-68] - BSA and Azide free (ab256352)
        Immunoprecipitation - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22639-68] - BSA and Azide free (ab256352)

        Asialoglycoprotein Receptor 1/HL-1 was immunoprecipitated from 0.35 mg human liver lysate with ab254261 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab254261 at 1/1000 dilution (0.45 μg/ml). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

        Lane 1: Human liver lysate 10μg.

        Lane 2: ab254261 IP in human liver lysate.

        Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab254261 in human liver lysate.

        Blocking and dilution buffer and concentration: 5% NFDM/TBST.

        Exposure time: 1 second.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254261).

      • Immunoprecipitation - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22639-68] - BSA and Azide free (ab256352)
        Immunoprecipitation - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22639-68] - BSA and Azide free (ab256352)

        Asialoglycoprotein Receptor 1/HL-1 was immunoprecipitated from 0.35 mg HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate with ab254261 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab254261 1/1000 dilution (0.45 μg/ml). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

        Lane 1: HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate 10μg
        Lane 2: ab254261 IP in HepG2 whole cell lysate
        Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab254261 in HepG2 whole cell lysate.

        Blocking and dilution buffer and concentration: 5% NFDM/TBST.

        Exposure time: 1 second

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254261).

      • Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22639-68] - BSA and Azide free (ab256352)
        Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22639-68] - BSA and Azide free (ab256352)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References (0)

      Publishing research using ab256352? Please let us know so that we can cite the reference in this datasheet.

      ab256352 has not yet been referenced specifically in any publications.

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