Recombinant
RabMAb

Recombinant Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22639-68] - BSA and Azide free (ab256352)

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Overview

  • Product name

    Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22639-68] - BSA and Azide free
    See all Asialoglycoprotein Receptor 1/HL-1 primary antibodies

  • Description

    Rabbit monoclonal [EPR22639-68] to Asialoglycoprotein Receptor 1/HL-1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, IHC-P, IPmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human Asialoglycoprotein Receptor 1/HL-1 aa 50-150. The exact sequence is proprietary.
    Database link: P07306

  • Positive control

    • WB: HepG2 and Huh7 cell lysates whole cell lysate. Human liver tissue lysate. IP: HepG2 whole cell lysates IHC-P Human liver and liver cancer tissue ICC/IF: HepG2 cells.

  • General notes

    Ab256352 is the carrier-free version of ab254261. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab256352 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Constituent: PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR22639-68

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab256352 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 33 kDa.
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for Flow Cyt.

    • Target

    • Function

      Mediates the endocytosis of plasma glycoproteins to which the terminal sialic acid residue on their complex carbohydrate moieties has been removed. The receptor recognizes terminal galactose and N-acetylgalactosamine units. After ligand binding to the receptor, the resulting complex is internalized and transported to a sorting organelle, where receptor and ligand are disassociated. The receptor then returns to the cell membrane surface.

    • Tissue specificity

      Expressed exclusively in hepatic parenchymal cells.

    • Sequence similarities

      Contains 1 C-type lectin domain.

    • Post-translational
      modifications

      Phosphorylated on a cytoplasmic Ser residue.

    • Cellular localization

      Membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • ASGP-R 1 antibody
        • ASGPR 1 antibody
        • ASGPR antibody
        • ASGPR1 antibody
        • Asgr1 antibody
        • ASGR1_HUMAN antibody
        • Asialoglycoprotein receptor 1 antibody
        • C type lectin domain family 4 member H1 antibody
        • C-type lectin domain family 4 member H1 antibody
        • CLEC4H1 antibody
        • Hepatic lectin H1 antibody
        • HL-1 antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22639-68] - BSA and Azide free (ab256352)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22639-68] - BSA and Azide free (ab256352)

        Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Asialoglycoprotein Receptor 1/HL-1 with ab254261 at 1/2000 dilution (0.25 μg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in human liver (PMID:23979840,26422581,27241665). Counterstained with hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

        Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254261).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22639-68] - BSA and Azide free (ab256352)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22639-68] - BSA and Azide free (ab256352)

        Immunohistochemical analysis of paraffin-embedded human liver cancer tissue labeling Asialoglycoprotein Receptor 1/HL-1 with ab254261 at 1/2000 dilution (0.25 μg/ml)  followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in human liver cancer (PMID:23979840,26422581,27241665). Counterstained with hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

        Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254261).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22639-68] - BSA and Azide free (ab256352)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22639-68] - BSA and Azide free (ab256352)

        Negative control: No staining in human colon (PMID:23979840).

        Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Asialoglycoprotein Receptor 1/HL-1 with ab254261 at 1/2000 dilution (0.25 μg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Counterstained with hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

        Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254261).

      • Immunocytochemistry/ Immunofluorescence - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22639-68] - BSA and Azide free (ab256352)
        Immunocytochemistry/ Immunofluorescence - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22639-68] - BSA and Azide free (ab256352)

        Immunofluorescent analysis of 100% Methanol-fixed HepG2 (human hepatocellular carcinoma epithelial cell) cells labeling Asialoglycoprotein Receptor 1/HL-1 with at 1/50 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in HepG2 cell line is observed. Methanol fixation is preferred.

        ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)  was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

        Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254261).

      • Immunoprecipitation - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22639-68] - BSA and Azide free (ab256352)
        Immunoprecipitation - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22639-68] - BSA and Azide free (ab256352)

        Asialoglycoprotein Receptor 1/HL-1 was immunoprecipitated from 0.35 mg human liver lysate with ab254261 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab254261 at 1/1000 dilution (0.45 μg/ml). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

        Lane 1: Human liver lysate 10μg.

        Lane 2: ab254261 IP in human liver lysate.

        Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab254261 in human liver lysate.

        Blocking and dilution buffer and concentration: 5% NFDM/TBST.

        Exposure time: 1 second.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254261).

      • Immunoprecipitation - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22639-68] - BSA and Azide free (ab256352)
        Immunoprecipitation - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22639-68] - BSA and Azide free (ab256352)

        Asialoglycoprotein Receptor 1/HL-1 was immunoprecipitated from 0.35 mg HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate with ab254261 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab254261 1/1000 dilution (0.45 μg/ml). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

        Lane 1: HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate 10μg
        Lane 2: ab254261 IP in HepG2 whole cell lysate
        Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab254261 in HepG2 whole cell lysate.

        Blocking and dilution buffer and concentration: 5% NFDM/TBST.

        Exposure time: 1 second

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254261).

      References

      ab256352 has not yet been referenced specifically in any publications.

      Publishing research using ab256352? Please let us know so that we can cite the reference in this datasheet.

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