Anti-ASIC1 antibody - C-terminal (ab176203)

Overview

  • Product name
    Anti-ASIC1 antibody - C-terminal
    See all ASIC1 primary antibodies
  • Description
    Rabbit polyclonal to ASIC1 - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken
  • Immunogen

    Synthetic peptide within Human ASIC1 aa 499-528 (C terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: P78348

  • Positive control
    • CEM cells and lysates; Human brain tissue.
  • General notes

    Protein previously known as ACCN2.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab176203 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 60 kDa.
Flow Cyt 1/10 - 1/100.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/50 - 1/100.

Target

  • Function
    Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Also permeable for Ca(2+), Li(+) and K(+). Generates a biphasic current with a fast inactivating and a slow sustained phase. Mediates glutamate-independent Ca(2+) entry into neurons upon acidosis. This Ca(2+) overloading is toxic for cortical neurons and may be in part responsible for ischemic brain injury. Heteromeric channel assembly seems to modulate channel properties. Functions as a postsynaptic proton receptor that influences intracellular Ca(2+) concentration and calmodulin-dependent protein kinase II phosphorylation and thereby the density of dendritic spines. Modulates activity in the circuits underlying innate fear.
  • Tissue specificity
    Expressed in most or all neurons.
  • Sequence similarities
    Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family. ACCN2 subfamily.
  • Post-translational
    modifications
    Phosphorylation by PKA regulates interaction with PRKCABP and subcellular location. Phosphorylation by PKC may regulate the channel.
  • Cellular localization
    Cell membrane. Localizes in synaptosomes at dendritic synapses of neurons. Colocalizes with DLG4.
  • Information by UniProt
  • Database links
  • Alternative names
    • ACCN2 antibody
    • ACCN2_HUMAN antibody
    • Acid sensing ion channel 1 antibody
    • Acid sensing ion channel 1a protein antibody
    • Acid sensing ion channel antibody
    • Acid-sensing ion channel 1 antibody
    • AI843610 antibody
    • Amiloride sensitive cation channel 2 neuronal antibody
    • Amiloride-sensitive cation channel 2 antibody
    • ASIC antibody
    • ASIC1 antibody
    • ASIC1A antibody
    • B530003N02Rik antibody
    • BNaC2 antibody
    • Brain sodium channel 2 antibody
    • Cation channel amiloride sensitive neuronal 2 antibody
    • hBNaC2 antibody
    • neuronal antibody
    see all

Images

  • Anti-ASIC1 antibody - C-terminal (ab176203) at 1/1000 dilution + CEM cell lysate at 35 µg

    Predicted band size: 60 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded human brain tissue labeling ASIC1 with ab176203 at 1/50 dilution, followed by peroxidase-conjugation to the secondary antibody and DAB staining.

  • Flow cytometric analysis of CEM cells labeling ASIC1 with ab176203 at 1/10 dilution (bottom histogram), compared to a negative control cell (top histogram). FITC-conjugated Goat-anti-Rabbit secondary antibodies were used for the analysis.

References

ab176203 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer (pH6)
Permeabilization
Yes - 0.1% Triton-X100
Specification
brain
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
Fixative
Paraformaldehyde
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Verified customer

Submitted Sep 17 2018

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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