Key features and details
- Mouse monoclonal [S271-44] to ASIC1
- Suitable for: WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG1
Product nameAnti-ASIC1 antibody [S271-44]
See all ASIC1 primary antibodies
DescriptionMouse monoclonal [S271-44] to ASIC1
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse
Fusion protein corresponding to Mouse ASIC1 aa 460-526 (C terminal).
Database link: Q6NXK8
- WB: Rat brain lysate. ICC/IF: SK-N-BE cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityProtein G purified
Purification notesPurified from TCS.
Our Abpromise guarantee covers the use of ab240896 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 60 kDa.|
FunctionCation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Also permeable for Ca(2+), Li(+) and K(+). Generates a biphasic current with a fast inactivating and a slow sustained phase. Mediates glutamate-independent Ca(2+) entry into neurons upon acidosis. This Ca(2+) overloading is toxic for cortical neurons and may be in part responsible for ischemic brain injury. Heteromeric channel assembly seems to modulate channel properties. Functions as a postsynaptic proton receptor that influences intracellular Ca(2+) concentration and calmodulin-dependent protein kinase II phosphorylation and thereby the density of dendritic spines. Modulates activity in the circuits underlying innate fear.
Tissue specificityExpressed in most or all neurons.
Sequence similaritiesBelongs to the amiloride-sensitive sodium channel (TC 1.A.6) family. ACCN2 subfamily.
modificationsPhosphorylation by PKA regulates interaction with PRKCABP and subcellular location. Phosphorylation by PKC may regulate the channel.
Cellular localizationCell membrane. Localizes in synaptosomes at dendritic synapses of neurons. Colocalizes with DLG4.
- Information by UniProt
- ACCN2 antibody
- ACCN2_HUMAN antibody
- Acid sensing ion channel 1 antibody
Lane 1 : Anti-ASIC1 antibody [S271-44] (ab240896) at 1/100 dilution
Lane 2 : Anti-ASIC1 antibody [S271-44] (ab240896) at 1/250 dilution
Lane 3 : Anti-ASIC1 antibody [S271-44] (ab240896) at 1/500 dilution
Lane 4 : Anti-ASIC1 antibody [S271-44] (ab240896) at 1/1000 dilution
All lanes : Rat brain lysate
Predicted band size: 60 kDa
Immunofluorescent analysis of 4% paraformaldehyde-fixed SK-N-BE (human neuroblastoma cell line) cells labeling ASIC1 with ab240896 at 1/100 dilution for 60 minutes at room temperature, followed by Goat Anti-Mouse ATTO 488 at 1/100 dilution for 60 minutes (green). The nuclear counter stain is DAPI (blue). Actin is detected with Phalloidin Texas Red F-Actin stain (red).
A: DAPI, B: Actin, C: ASIC1, D: Composite.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab240896 has not yet been referenced specifically in any publications.