Anti-ASIC1 antibody [S271-44] (ab240896)
![Western blot - Anti-ASIC1 antibody [S271-44] (ab240896)](https://www.abcam.com/ps/products/240/ab240896/Images/ab240896-319722-anti-asic1-antibody-s271-44-western-blot.jpg "Western blot - Anti-ASIC1 antibody [S271-44] (ab240896)")
Key features and details
- Mouse monoclonal [S271-44] to ASIC1
- Suitable for: WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-ASIC1 antibody [S271-44]
See all ASIC1 primary antibodies -
Description
Mouse monoclonal [S271-44] to ASIC1 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse
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Immunogen
Fusion protein corresponding to Mouse ASIC1 aa 460-526 (C terminal).
Database link: Q6NXK8 -
Positive control
- WB: Rat brain lysate. ICC/IF: SK-N-BE cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
S271-44 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab240896 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Predicted molecular weight: 60 kDa. | |
| ICC/IF | 1/100. |
Target
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Function
Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Also permeable for Ca(2+), Li(+) and K(+). Generates a biphasic current with a fast inactivating and a slow sustained phase. Mediates glutamate-independent Ca(2+) entry into neurons upon acidosis. This Ca(2+) overloading is toxic for cortical neurons and may be in part responsible for ischemic brain injury. Heteromeric channel assembly seems to modulate channel properties. Functions as a postsynaptic proton receptor that influences intracellular Ca(2+) concentration and calmodulin-dependent protein kinase II phosphorylation and thereby the density of dendritic spines. Modulates activity in the circuits underlying innate fear. -
Tissue specificity
Expressed in most or all neurons. -
Sequence similarities
Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family. ACCN2 subfamily. -
Post-translational
modificationsPhosphorylation by PKA regulates interaction with PRKCABP and subcellular location. Phosphorylation by PKC may regulate the channel. -
Cellular localization
Cell membrane. Localizes in synaptosomes at dendritic synapses of neurons. Colocalizes with DLG4. - Information by UniProt
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Database links
- Entrez Gene: 41 Human
- Entrez Gene: 11419 Mouse
- Entrez Gene: 79123 Rat
- Omim: 602866 Human
- SwissProt: P78348 Human
- SwissProt: Q6NXK8 Mouse
- SwissProt: P55926 Rat
- Unigene: 274361 Human
see all -
Alternative names
- ACCN2 antibody
- ACCN2_HUMAN antibody
- Acid sensing ion channel 1 antibody
see all
Images
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Western blot - Anti-ASIC1 antibody [S271-44] (ab240896)Lane 1 : Anti-ASIC1 antibody [S271-44] (ab240896) at 1/100 dilution
Lane 2 : Anti-ASIC1 antibody [S271-44] (ab240896) at 1/250 dilution
Lane 3 : Anti-ASIC1 antibody [S271-44] (ab240896) at 1/500 dilution
Lane 4 : Anti-ASIC1 antibody [S271-44] (ab240896) at 1/1000 dilution
All lanes : Rat brain lysate
Predicted band size: 60 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-ASIC1 antibody [S271-44] (ab240896)](https://www.abcam.com/ps/products/240/ab240896/Images/ab240896-319723-anti-asic1-antibody-s271-44-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ASIC1 antibody [S271-44] (ab240896)Immunofluorescent analysis of 4% paraformaldehyde-fixed SK-N-BE (human neuroblastoma cell line) cells labeling ASIC1 with ab240896 at 1/100 dilution for 60 minutes at room temperature, followed by Goat Anti-Mouse ATTO 488 at 1/100 dilution for 60 minutes (green). The nuclear counter stain is DAPI (blue). Actin is detected with Phalloidin Texas Red F-Actin stain (red).
A: DAPI, B: Actin, C: ASIC1, D: Composite.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab240896 has not yet been referenced specifically in any publications.
