Recombinant
Recombinant Anti-ASIC2 antibody [EPR11029] (ab169768)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-ASIC2 antibody [EPR11029]
See all ASIC2 primary antibodies -
Description
Rabbit monoclonal [EPR11029] to ASIC2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human ASIC2 aa 450-550 (Cysteine residue).
Database link: Q16515 -
Positive control
- Human fetal kidney and Human glioma lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11029 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab169768 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/5000. Predicted molecular weight: 58 kDa. |
Application notes
Is unsuitable for ICC,IHC-P or IP.
Target
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Function
Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Also permeable for Li(+) and K(+). Generates a biphasic current with a fast inactivating and a slow sustained phase. Heteromeric channel assembly seems to modulate. -
Tissue specificity
Brain and spinal cord. Isoform 1 is also detected in testis, liver, colon and ovary. -
Sequence similarities
Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family. ACCN1 subfamily. -
Cellular localization
Cell membrane. Localized at the plasma membrane of neurons, in the soma and punctated peripheral processes. - Information by UniProt
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Database links
- Entrez Gene: 40 Human
- Entrez Gene: 11418 Mouse
- Entrez Gene: 25364 Rat
- Omim: 601784 Human
- SwissProt: Q16515 Human
- SwissProt: Q925H0 Mouse
- SwissProt: Q62962 Rat
- Unigene: 368417 Human
see all -
Alternative names
- ACCN antibody
- ACCN1 antibody
- ACCN1_HUMAN antibody
see all
Images
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![Western blot - Anti-ASIC2 antibody [EPR11029] (ab169768)](https://www.abcam.com/ps/products/169/ab169768/Images/ab169768-168431-anti-asic2-antibody-epr11029-western-blot.jpg)
Western blot - Anti-ASIC2 antibody [EPR11029] (ab169768)All lanes : Anti-ASIC2 antibody [EPR11029] (ab169768) at 1/1000 dilution
Lane 1 : Human fetal kidney lysate
Lane 2 : Human glioma lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 58 kDa
Protocols
Datasheets and documents
References
This product has been referenced in:
- Zhang H et al. Glucose Deficiency Elevates Acid-Sensing Ion Channel 2a Expression and Increases Seizure Susceptibility in Temporal Lobe Epilepsy. Sci Rep 7:5870 (2017). Read more (PubMed: 28725010) »
