Recombinant Anti-ASIC2 antibody [EPR11029] (ab169768)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11029] to ASIC2
- Suitable for: WB
- Reacts with: Human
Overview
-
Product name
Anti-ASIC2 antibody [EPR11029]
See all ASIC2 primary antibodies -
Description
Rabbit monoclonal [EPR11029] to ASIC2 -
Host species
Rabbit -
Tested Applications & Species
Application Species WB Human -
Immunogen
Synthetic peptide corresponding to Human ASIC2 aa 450-550 (Cysteine residue).
Database link: Q16515 -
Positive control
- Human fetal kidney and Human glioma lysates.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
-
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11029 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab169768 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
---|---|
ICC |
Human
|
IHC-P |
Mouse
|
WB |
Human
|
All applications |
Mouse
Rat
|
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/5000. Predicted molecular weight: 58 kDa.
|
Notes |
---|
WB
1/1000 - 1/5000. Predicted molecular weight: 58 kDa. |
Target
-
Function
Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Also permeable for Li(+) and K(+). Generates a biphasic current with a fast inactivating and a slow sustained phase. Heteromeric channel assembly seems to modulate. -
Tissue specificity
Brain and spinal cord. Isoform 1 is also detected in testis, liver, colon and ovary. -
Sequence similarities
Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family. ACCN1 subfamily. -
Cellular localization
Cell membrane. Localized at the plasma membrane of neurons, in the soma and punctated peripheral processes. - Information by UniProt
-
Database links
- Entrez Gene: 40 Human
- Entrez Gene: 11418 Mouse
- Entrez Gene: 25364 Rat
- Omim: 601784 Human
- SwissProt: Q16515 Human
- SwissProt: Q925H0 Mouse
- SwissProt: Q62962 Rat
- Unigene: 368417 Human
see all -
Alternative names
- ACCN antibody
- ACCN1 antibody
- ACCN1_HUMAN antibody
see all
Images
-
All lanes : Anti-ASIC2 antibody [EPR11029] (ab169768) at 1/1000 dilution
Lane 1 : Human fetal kidney lysate
Lane 2 : Human glioma lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 58 kDa
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab169768 has been referenced in 1 publication.
- Zhang H et al. Glucose Deficiency Elevates Acid-Sensing Ion Channel 2a Expression and Increases Seizure Susceptibility in Temporal Lobe Epilepsy. Sci Rep 7:5870 (2017). PubMed: 28725010