RecombinantRabMAb

Recombinant Anti-ASIC2 antibody [EPR11029] (ab169768)

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Western blot - Anti-ASIC2 antibody [EPR11029] (ab169768)
  • Anti-ASIC2 antibody [EPR11029] (ab169768)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11029] to ASIC2
  • Suitable for: WB
  • Reacts with: Human

Overview

  • Product name

    Anti-ASIC2 antibody [EPR11029]
    See all ASIC2 primary antibodies

  • Description

    Rabbit monoclonal [EPR11029] to ASIC2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Human ASIC2 aa 450-550 (Cysteine residue).
    Database link: Q16515

  • Positive control

    • Human fetal kidney and Human glioma lysates.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR11029

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab169768 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC
Human
IHC-P
Mouse
WB
Human
All applications
Mouse
Rat
Application Abreviews Notes
WB
1/1000 - 1/5000. Predicted molecular weight: 58 kDa.
Notes
WB
1/1000 - 1/5000. Predicted molecular weight: 58 kDa.
  • Application notes
    Is unsuitable for ICC,IHC-P or IP.

    • Target

    • Function

      Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Also permeable for Li(+) and K(+). Generates a biphasic current with a fast inactivating and a slow sustained phase. Heteromeric channel assembly seems to modulate.

    • Tissue specificity

      Brain and spinal cord. Isoform 1 is also detected in testis, liver, colon and ovary.

    • Sequence similarities

      Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family. ACCN1 subfamily.

    • Cellular localization

      Cell membrane. Localized at the plasma membrane of neurons, in the soma and punctated peripheral processes.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • ACCN antibody
      • ACCN1 antibody
      • ACCN1_HUMAN antibody
      • Acid sensing ion channel 2 antibody
      • Acid-sensing ion channel 2 antibody
      • Amiloride sensitive brain sodium channel antibody
      • Amiloride sensitive cation channel 1 neuronal (degenerin) antibody
      • Amiloride sensitive cation channel 1 neuronal antibody
      • Amiloride-sensitive brain sodium channel antibody
      • Amiloride-sensitive cation channel 1, neuronal antibody
      • Amiloride-sensitive cation channel neuronal 1 antibody
      • ASIC2 antibody
      • ASIC2a antibody
      • BNaC1 antibody
      • BNC1 antibody
      • Brain sodium channel 1 antibody
      • Degenerin antibody
      • hBNaC1 antibody
      • Mammalian degenerin homolog antibody
      • MDEG antibody
      • Neuronal amiloride sensitive cation channel 1 antibody
      see all

    Images

    • Western blot - Anti-ASIC2 antibody [EPR11029] (ab169768)
      Western blot - Anti-ASIC2 antibody [EPR11029] (ab169768)
      All lanes : Anti-ASIC2 antibody [EPR11029] (ab169768) at 1/1000 dilution

      Lane 1 : Human fetal kidney lysate
      Lane 2 : Human glioma lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 58 kDa

    • Anti-ASIC2 antibody [EPR11029] (ab169768)
      Anti-ASIC2 antibody [EPR11029] (ab169768)

    References (1)

    Publishing research using ab169768? Please let us know so that we can cite the reference in this datasheet.

    ab169768 has been referenced in 1 publication.

    • Zhang H  et al. Glucose Deficiency Elevates Acid-Sensing Ion Channel 2a Expression and Increases Seizure Susceptibility in Temporal Lobe Epilepsy. Sci Rep 7:5870 (2017). PubMed: 28725010

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