Key features and details
- Rabbit polyclonal to ASIC3
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ASIC3 antibody
See all ASIC3 primary antibodies
DescriptionRabbit polyclonal to ASIC3
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Cat, Pig
- Fetal kidney lysate; human kidney tissue
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab49333 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 2.5 µg/ml. Predicted molecular weight: 59 kDa.
Dilute in PBS or medium which is identical to that used in the assay system. At 100 nM, this antibody inhibits 50% of Factor XII activity. This antibody can also be used in a coagulation assay. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
|IHC-P||Use a concentration of 4 - 8 µg/ml.|
FunctionCation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Generates a biphasic current with a fast inactivating and a slow sustained phase. In sensory neurons is proposed to mediate the pain induced by acidosis that occurs in ischemic, damaged or inflamed tissue. May be involved in hyperalgesia. May play a role in mechanoreception. Heteromeric channel assembly seems to modulate channel properties.
Tissue specificityExpressed by sensory neurons. Strongly expressed in brain, spinal chord, lung, lymph nodes, kidney, pituitary, heart and testis.
Sequence similaritiesBelongs to the amiloride-sensitive sodium channel (TC 1.A.6) family. ACCN3 subfamily.
Developmental stageExpressed in fetal tissues, expression increases in lung and kidney adult tissues.
DomainThe PDZ domain-binding motif is involved in interaction with LIN7A, GOPC and MAGI1.
modificationsPhosphorylated by PKA. Phosphorylated by PKC. In vitro, PRKCABP/PICK-1 is necessary for PKC phosphorylation and activation of a ACCN3/ASIC3-ACCN1/ASIC2b channel, but does not activate a homomeric ACCN3 channel.
Cellular localizationCell membrane. Cytoplasm. Cell surface expression may be stabilized by interaction with LIN7B and cytoplasmic retention by interaction with DLG4. In part cytoplasmic in cochlea cells.
- Information by UniProt
- ACCN 3 antibody
- Accn3 antibody
- ACCN3_HUMAN antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin embedded sections) analysis of human kidney tissue labelling ASIC3 with ab49333 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of renal tubules. Magnification: 400X.
Anti-ASIC3 antibody (ab49333) at 2.5 µg/ml + Jurkat cell lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 59 kDa
Observed band size: 59 kDa
Gel concentration 12%
ab49333 has been referenced in 3 publications.
- Detweiler ND et al. Loss of acid-sensing ion channel 2 enhances pulmonary vascular resistance and hypoxic pulmonary hypertension. J Appl Physiol (1985) 127:393-407 (2019). PubMed: 31169471
- Wang D et al. Upregulation of nuclear factor-?B and acid sensing ion channel 3 in dorsal root ganglion following application of nucleus pulposus onto the nerve root in rats. Mol Med Rep 16:4309-4314 (2017). PubMed: 28765874
- Gilbert HT et al. Acidic pH promotes intervertebral disc degeneration: Acid-sensing ion channel -3 as a potential therapeutic target. Sci Rep 6:37360 (2016). WB, IF ; Human . PubMed: 27853274