Overview

  • Product name

  • Description

    Rabbit polyclonal to ASK1
  • Host species

    Rabbit
  • Specificity

    This antibody detects endogenous levels of total ASK1 protein
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide from around amino acids 964-968 (

    SISLP

    ) of Human ASK1 (NP_005914.1)

  • Positive control

    • HeLa cells, 293 cell extract, Human breast carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab131506 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 155 kDa.
IHC-P 1/50 - 1/100.
ICC/IF 1/100 - 1/200.

Target

  • Function

    Component of a protein kinase signal transduction cascade. Phosphorylates and activates MAP2K4 and MAP2K6, which in turn activate the JNK and p38 MAP kinases, respectively. Overexpression induces apoptotic cell death.
  • Tissue specificity

    Abundantly expressed in heart and pancreas.
  • Sequence similarities

    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Dephosphorylated and activated by PGAM5.
  • Information by UniProt
  • Database links

  • Alternative names

    • Apoptosis signal regulating kinase 1 antibody
    • Apoptosis signal-regulating kinase 1 antibody
    • ASK 1 antibody
    • ASK-1 antibody
    • ASK1 antibody
    • M3K5 antibody
    • M3K5_HUMAN antibody
    • MAP/ERK kinase kinase 5 antibody
    • MAP3K5 antibody
    • MAPK/ERK kinase kinase 5 antibody
    • MAPKKK5 antibody
    • MEK kinase 5 antibody
    • MEKK 5 antibody
    • MEKK5 antibody
    • Mitogen activated protein kinase kinase kinase 5 antibody
    • Mitogen-activated protein kinase kinase kinase 5 antibody
    see all

Images

  • All lanes : Anti-ASK1 antibody (ab131506) at 1/500 dilution

    Lane 1 : 293 cell extract
    Lane 2 : HeLa cell extract

    Predicted band size: 155 kDa

  • Immunofluorescence analysis of methanol fixed HeLa cells labelled with ab131506 at 1/100 dilution.
  • Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelled with ab131506 at 1/50 dilution (left) and ab131506 preincubated with blocking peptide (right).

References

This product has been referenced in:

  • Zhang L  et al. Thioredoxin-1 Protects Bone Marrow-Derived Mesenchymal Stromal Cells from Hyperoxia-Induced Injury In Vitro. Oxid Med Cell Longev 2018:1023025 (2018). Read more (PubMed: 29599892) »
  • Gao J  et al. LncRNA-NR_033515 promotes proliferation, fibrogenesis and epithelial-to-mesenchymal transition by targeting miR-743b-5p in diabetic nephropathy. Biomed Pharmacother 106:543-552 (2018). Read more (PubMed: 29990842) »
See all 6 Publications for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab131506.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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