Western blot abreview for Anti-ASK1 antibody [EP553Y]

Excellent
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Bone-marrow derived dendritic cells)
Loading amount
70 µg
Specification
Bone-marrow derived dendritic cells
Gel Running Conditions
Reduced Denaturing (8% SDS gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/500
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA

Secondary antibody

Name
Non-Abcam antibody was used: Donkey anti-Rabbit IgG (H+L)
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/20000

Detection

Detection method
ECL+
Exposure
6 minute(s) and 0 second(s)
Bands
Specific: 155 kDa Non-specific: 110, 40 kDa

Additional data

Additional Notes
12 min. exposure or increased/decreased dilutions of 1st and 2nd antibody can be used to detect ASK1 in even lower amount of total cell lysates.

Dr. Caglar Cekic

Verified customer

Submitted May 13 2009

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