Western blot abreview for Anti-ASK1 antibody [EP553Y]

Average
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (neuron)
Loading amount
20 µg
Specification
neuron
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C

Other product details

Dilution
1/2000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C

Secondary antibody

Name
Non-Abcam antibody was used: goat anti rabbit antibody
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Detection

Detection method
ECL
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 150 kDa

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Submitted Jun 03 2008

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