ASK1 overexpression 293T lysate (whole cell) (ab94062)
Overview
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Product name
ASK1 overexpression 293T lysate (whole cell)
See all ASK1 lysates -
General notes
ab94062 is a 293T cell transfected lysate in which Human ASK1 has been transiently over-expressed using a pCMV-ASK1 plasmid. The lysate is provided in 1X Sample Buffer.
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Tested applications
Suitable for: WBmore details
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
Storage buffer
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Research areas
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Background
Function: Component of a protein kinase signal transduction cascade. Phosphorylates and activates MAP2K4 and MAP2K6, which in turn activate the JNK and p38 MAP kinases, respectively. Overexpression induces apoptotic cell death. Tissue specificity: Abundantly expressed in heart and pancreas. Similarity: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily. Contains 1 protein kinase domain. PTM: Dephosphorylated and activated by PGAM5.
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab94062 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent dilution.
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Notes |
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WB
Use at an assay dependent dilution. |
Images
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ab94062 at 15µg/lane on an SDS-PAGE gel.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab94062 has not yet been referenced specifically in any publications.