Product nameASK1 overexpression 293T lysate (whole cell)
ab94062 is a 293T cell transfected lysate in which Human ASK1 has been transiently over-expressed using a pCMV-ASK1 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferpH: 6.80
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% SDS, 0.788% Tris HCl, 10% Glycerol
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BackgroundFunction: Component of a protein kinase signal transduction cascade. Phosphorylates and activates MAP2K4 and MAP2K6, which in turn activate the JNK and p38 MAP kinases, respectively. Overexpression induces apoptotic cell death. Tissue specificity: Abundantly expressed in heart and pancreas. Similarity: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily. Contains 1 protein kinase domain. PTM: Dephosphorylated and activated by PGAM5.
Our Abpromise guarantee covers the use of ab94062 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
ab94062 at 15µg/lane on an SDS-PAGE gel.
ab94062 has not yet been referenced specifically in any publications.