Overview

Description

  • Nature
    Synthetic

Associated products

Specifications

Our Abpromise guarantee covers the use of ab224650 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Blocking - Blocking peptide for Anti-ASK1 antibody [EP553Y] (ab45178)

  • Form
    Lyophilised
  • Additional notes

    - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.

    - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.

    - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.

    - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.

    - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C.

General Info

  • Alternative names
    • Apoptosis signal regulating kinase 1
    • Apoptosis signal-regulating kinase 1
    • ASK 1
    • ASK-1
    • ASK1
    • M3K5
    • M3K5_HUMAN
    • MAP/ERK kinase kinase 5
    • MAP3K5
    • MAPK/ERK kinase kinase 5
    • MAPKKK5
    • MEK kinase 5
    • MEKK 5
    • MEKK5
    • Mitogen activated protein kinase kinase kinase 5
    • Mitogen-activated protein kinase kinase kinase 5
    see all
  • Function
    Component of a protein kinase signal transduction cascade. Phosphorylates and activates MAP2K4 and MAP2K6, which in turn activate the JNK and p38 MAP kinases, respectively. Overexpression induces apoptotic cell death.
  • Tissue specificity
    Abundantly expressed in heart and pancreas.
  • Sequence similarities
    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Dephosphorylated and activated by PGAM5.
  • Information by UniProt

References

ab224650 has not yet been referenced specifically in any publications.

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