Overview

  • Product name

    Anti-ASK1 (phospho S83) antibody
    See all ASK1 primary antibodies
  • Description

    Rabbit polyclonal to ASK1 (phospho S83)
  • Host species

    Rabbit
  • Specificity

    ab47304 detects endogenous levels of ASK1 only when phosphorylated at serine 83.
  • Tested applications

    Suitable for: WB, IHC-P, ELISAmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    synthesized phosphopeptide derived from human ASK1 around the phosphorylation site of serine 83 (G-S-SP-V-G)

  • Positive control

    • Human breast carcinoma tissue and TNF alpha stimulated K562 cell extracts

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    The antibody was purified using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab47304 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 155 kDa (predicted molecular weight: 155 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/20000.

Target

  • Function

    Component of a protein kinase signal transduction cascade. Phosphorylates and activates MAP2K4 and MAP2K6, which in turn activate the JNK and p38 MAP kinases, respectively. Overexpression induces apoptotic cell death.
  • Tissue specificity

    Abundantly expressed in heart and pancreas.
  • Sequence similarities

    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Dephosphorylated and activated by PGAM5.
  • Information by UniProt
  • Database links

  • Alternative names

    • Apoptosis signal regulating kinase 1 antibody
    • Apoptosis signal-regulating kinase 1 antibody
    • ASK 1 antibody
    • ASK-1 antibody
    • ASK1 antibody
    • M3K5 antibody
    • M3K5_HUMAN antibody
    • MAP/ERK kinase kinase 5 antibody
    • MAP3K5 antibody
    • MAPK/ERK kinase kinase 5 antibody
    • MAPKKK5 antibody
    • MEK kinase 5 antibody
    • MEKK 5 antibody
    • MEKK5 antibody
    • Mitogen activated protein kinase kinase kinase 5 antibody
    • Mitogen-activated protein kinase kinase kinase 5 antibody
    see all

Images

  • ab47304 staining human breast carcinoma tissue by IHC-P (left hand panel). The right hand panel shows staining in the presence of phospho-peptide.
  • All lanes : Anti-ASK1 (phospho S83) antibody (ab47304)

    Lane 1 : TNF alpha stimulated K562 cells
    Lane 2 : K562 cells

    Predicted band size: 155 kDa

References

This product has been referenced in:

  • Liangpunsakul S  et al. Imipramine blocks ethanol-induced ASMase activation, ceramide generation, and PP2A activation, and ameliorates hepatic steatosis in ethanol-fed mice. Am J Physiol Gastrointest Liver Physiol 302:G515-23 (2012). Read more (PubMed: 22194417) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (skin Tumor tissue)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pepsin solution(GBI LAB)
Permeabilization
No
Specification
skin Tumor tissue
Blocking step
1% BSA with 10% normal serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 8°C
Fixative
Paraformaldehyde

Dr. Sung Un Kang

Verified customer

Submitted Oct 24 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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