Recombinant Anti-ASMT antibody [EPR12458] (ab180511)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12458] to ASMT
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-ASMT antibody [EPR12458]
See all ASMT primary antibodies -
Description
Rabbit monoclonal [EPR12458] to ASMT -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human ASMT aa 100-200 (internal sequence). The exact sequence is proprietary.
Database link: P46597 -
Positive control
- Y79 cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12458 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab180511 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/2000. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa). |
Target
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Tissue specificity
Most abundant in pineal gland, less in retina and brain, not in pigment epithelium. -
Pathway
Aromatic compound metabolism; melatonin biosynthesis; melatonin from serotonin: step 1/2. -
Sequence similarities
Belongs to the methyltransferase superfamily. - Information by UniProt
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Database links
- Entrez Gene: 438 Human
- Omim: 300015 Human
- Omim: 402500 Human
- SwissProt: P46597 Human
- Unigene: 522572 Human
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Alternative names
- acetylserotonin methyltransferase (Y chromosome) antibody
- acetylserotonin N-methyltransferase antibody
- Acetylserotonin O-methyltransferase antibody
see all
Images
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![Western blot - Anti-ASMT antibody [EPR12458] (ab180511)](https://www.abcam.com/ps/products/180/ab180511/Images/ab180511-211011-anti-asmt-antibody-epr12458-western-blot.jpg)
Western blot - Anti-ASMT antibody [EPR12458] (ab180511)Anti-ASMT antibody [EPR12458] (ab180511) at 1/1000 dilution + Y79 cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 38 kDa5% NFDM/TBST used as blocking and dilution buffer.
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![Anti-ASMT antibody [EPR12458] (ab180511)](https://www.abcam.com/ps/products/180/ab180511/Images/ab180511-2-benefits-of-recombinant-antibodies.png)
Anti-ASMT antibody [EPR12458] (ab180511)
Datasheets and documents
Certificate of Compliance
References (3)
ab180511 has been referenced in 3 publications.
- Wang Z et al. Photoperiod Affects Harderian Gland Morphology and Secretion in Female Cricetulus barabensis: Autophagy, Apoptosis, and Mitochondria. Front Physiol 11:408 (2020). PubMed: 32435203
- Ikegame M et al. Melatonin is a potential drug for the prevention of bone loss during space flight. J Pineal Res 67:e12594 (2019). PubMed: 31286565
- Chen HL et al. Restoration of hydroxyindole O-methyltransferase levels in human cancer cells induces a tryptophan-metabolic switch and attenuates cancer progression. J Biol Chem 293:11131-11142 (2018). PubMed: 29794137
