Anti-ASN antibody (ab243206)

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Overview

  • Product name

    Anti-ASN antibody

  • Description

    Rabbit polyclonal to ASN

  • Host species

    Rabbit

  • Specificity

    This antibody recognizes plant ASN2 and ASN1 isoproteins.

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Arabidopsis thaliana

  • Immunogen

    Recombinant full length protein corresponding to Arabidopsis thaliana ASN. (Purified ASN2).
    Database link: Q9LV77

  • Positive control

    • WB: Arabidopsis leaf extract.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Constituents: PBS, 50% Glycerol
  • Concentration information loading...

  • Purity

    Protein A/G purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab243206 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB 1/1000 - 1/2000. Predicted molecular weight: 65 kDa.

Target

  • Relevance

    Asparagine synthetase 2 (ASN2) is essential for nitrogen assimilation, distribution and remobilization within the plant via the phloem. ASN2 is expressed in leaf and ASN1 is expressed in floral organs. The amino acid sequences of Arabidopsis ASN1 and ASN2 are 76% identical. The amino acid sequences of Arabidopsis and Maize ASN2 are 73.6% identical.

  • Database links

    Images

    • Western blot - Anti-ASN antibody (ab243206)
      Western blot - Anti-ASN antibody (ab243206)
      Anti-ASN antibody (ab243206) at 1/1000 dilution + Arabidopsis leaf extract at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

      Predicted band size: 65 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASN antibody (ab243206)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASN antibody (ab243206)

      Arabidopsis thin leaf section was subjected to indirect immunofluorescence analysis using the Anti-ASN antibody as the primary antibody. Goat anti-rabbit IgG labelled with Alexa 405 was used as a secondary antibody. Blue: ASN protein probed with anti-ASN antibody Red: Autofluorescence

    References

    This product has been referenced in:

    • Gaufichon L  et al. ASN1-encoded asparagine synthetase in floral organs contributes to nitrogen filling in Arabidopsis seeds. Plant J 91:371-393 (2017). WB, IHC-P ; Arabidopsis thaliana . Read more (PubMed: 28390103) »
    • Gaufichon L  et al. Arabidopsis thaliana ASN2 encoding asparagine synthetase is involved in the control of nitrogen assimilation and export during vegetative growth. Plant Cell Environ 36:328-42 (2013). WB, IHC-P ; Arabidopsis thaliana . Read more (PubMed: 22789031) »
    See all 2 Publications for this product

    Publishing research using ab243206? Please let us know so that we can cite the reference in this datasheet.

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