Anti-ASN antibody (ab243206)
Key features and details
- Rabbit polyclonal to ASN
- Suitable for: IHC-P, WB
- Reacts with: Arabidopsis thaliana
- Isotype: IgG
Overview
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Product name
Anti-ASN antibody -
Description
Rabbit polyclonal to ASN -
Host species
Rabbit -
Specificity
This antibody recognizes plant ASN2 and ASN1 isoproteins.
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Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Arabidopsis thaliana -
Immunogen
Recombinant full length protein corresponding to Arabidopsis thaliana ASN. (Purified ASN2).
Database link: Q9LV77 -
Positive control
- WB: Arabidopsis leaf extract.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A/G purified -
Clonality
Polyclonal -
Isotype
IgG
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab243206 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use at an assay dependent concentration. | |
| WB | 1/1000 - 1/2000. Predicted molecular weight: 65 kDa. |
Target
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Relevance
Asparagine synthetase 2 (ASN2) is essential for nitrogen assimilation, distribution and remobilization within the plant via the phloem. ASN2 is expressed in leaf and ASN1 is expressed in floral organs. The amino acid sequences of Arabidopsis ASN1 and ASN2 are 76% identical. The amino acid sequences of Arabidopsis and Maize ASN2 are 73.6% identical. -
Database links
- SwissProt: Q9LV77 Arabidopsis thaliana
Images
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Western blot - Anti-ASN antibody (ab243206)Anti-ASN antibody (ab243206) at 1/1000 dilution + Arabidopsis leaf extract at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Predicted band size: 65 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASN antibody (ab243206)Arabidopsis thin leaf section was subjected to indirect immunofluorescence analysis using the Anti-ASN antibody as the primary antibody. Goat anti-rabbit IgG labelled with Alexa 405 was used as a secondary antibody. Blue: ASN protein probed with anti-ASN antibody Red: Autofluorescence
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (2)
ab243206 has been referenced in 2 publications.
- Gaufichon L et al. ASN1-encoded asparagine synthetase in floral organs contributes to nitrogen filling in Arabidopsis seeds. Plant J 91:371-393 (2017). WB, IHC-P ; Arabidopsis thaliana . PubMed: 28390103
- Gaufichon L et al. Arabidopsis thaliana ASN2 encoding asparagine synthetase is involved in the control of nitrogen assimilation and export during vegetative growth. Plant Cell Environ 36:328-42 (2013). WB, IHC-P ; Arabidopsis thaliana . PubMed: 22789031
