Key features and details
- Rabbit polyclonal to ASPA
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-ASPA antibody
See all ASPA primary antibodies
DescriptionRabbit polyclonal to ASPA
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment corresponding to a region within amino acids 1-313 of Human ASPA (Uniprot ID P45381).
- Mouse brain whole cell lysate. HeLa cells.
General notesKeep as concentrated solution.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab154503 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000 - 1/20000. Predicted molecular weight: 36 kDa.|
|ICC/IF||1/100 - 1/1000.|
FunctionCatalyzes the deacetylation of N-acetylaspartic acid (NAA) to produce acetate and L-aspartate. NAA occurs in high concentration in brain and its hydrolysis NAA plays a significant part in the maintenance of intact white matter. In other tissues it act as a scavenger of NAA from body fluids.
Tissue specificityBrain white matter, skeletal muscle, kidney, adrenal glands, lung and liver.
Involvement in diseaseDefects in ASPA are the cause of Canavan disease (CAND) [MIM:271900]; also known as spongy degeneration of the brain. CAND is a rare neurodegenerative condition of infancy or childhood characterized by white matter vacuolization and demeylination that gives rise to a spongy appearance. The clinical features are onset in early infancy, atonia of neck muscles, hypotonia, hyperextension of legs and flexion of arms, blindness, severe mental defect, megalocephaly, and death by 18 months on the average.
Sequence similaritiesBelongs to the AspA/AstE family. Aspartoacylase subfamily.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- ACY 2 antibody
- ACY-2 antibody
- ACY2 antibody
Anti-ASPA antibody (ab154503) at 1/10000 dilution + Mouse brain whole cell lysate at 20 µg/ml
Predicted band size: 36 kDa
10% SDS PAGE
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells labeling ASPA using ab154503 antibody at 1/500 dilution. Lower panel shows costaining with Hoechst 33342.
ab154503 has been referenced in 1 publication.
- Carpinelli MR et al. A new mouse model of Canavan leukodystrophy displays hearing impairment due to central nervous system dysmyelination. Dis Model Mech 7:649-57 (2014). WB ; Mouse . PubMed: 24682784