Anti-ASPA antibody (ab97454)
Key features and details
- Rabbit polyclonal to ASPA
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ASPA antibody
See all ASPA primary antibodies -
Description
Rabbit polyclonal to ASPA -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Cow, Pig
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Immunogen
Recombinant fragment, corresponding to a region within amino acids 39-270 of Human ASPA (AAH29128).
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab97454 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 36 kDa. | |
| IHC-P | 1/100 - 1/500. |
Target
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Function
Catalyzes the deacetylation of N-acetylaspartic acid (NAA) to produce acetate and L-aspartate. NAA occurs in high concentration in brain and its hydrolysis NAA plays a significant part in the maintenance of intact white matter. In other tissues it act as a scavenger of NAA from body fluids. -
Tissue specificity
Brain white matter, skeletal muscle, kidney, adrenal glands, lung and liver. -
Involvement in disease
Defects in ASPA are the cause of Canavan disease (CAND) [MIM:271900]; also known as spongy degeneration of the brain. CAND is a rare neurodegenerative condition of infancy or childhood characterized by white matter vacuolization and demeylination that gives rise to a spongy appearance. The clinical features are onset in early infancy, atonia of neck muscles, hypotonia, hyperextension of legs and flexion of arms, blindness, severe mental defect, megalocephaly, and death by 18 months on the average. -
Sequence similarities
Belongs to the AspA/AstE family. Aspartoacylase subfamily. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 509432 Cow
- Entrez Gene: 443 Human
- Entrez Gene: 11484 Mouse
- Entrez Gene: 100142661 Pig
- Omim: 608034 Human
- SwissProt: P45381 Human
- SwissProt: Q8R3P0 Mouse
- Unigene: 171142 Human
see all -
Alternative names
- ACY 2 antibody
- ACY-2 antibody
- ACY2 antibody
see all
Images
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Western blot - Anti-ASPA antibody (ab97454)All lanes : Anti-ASPA antibody (ab97454) at 1/1000 dilution
Lane 1 : MOLT4 whole cell lysate
Lane 2 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 36 kDa
10% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASPA antibody (ab97454)ab97454 at 1/100 dilution staining ASPA in paraffin-embedded Cal27 xenograft by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Protocols
References (1)
ab97454 has been referenced in 1 publication.
- Gessler DJ et al. Redirecting N-acetylaspartate metabolism in the central nervous system normalizes myelination and rescues Canavan disease. JCI Insight 2:e90807 (2017). PubMed: 28194442
