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Signal Transduction Metabolism Amino Acids
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Anti-ASPA antibody (ab97454)

  • Datasheet
  • SDS
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Western blot - Anti-ASPA antibody (ab97454)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASPA antibody (ab97454)

Key features and details

  • Rabbit polyclonal to ASPA
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-ASPA antibody
    See all ASPA primary antibodies
  • Description

    Rabbit polyclonal to ASPA
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Cow, Pig
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 39-270 of Human ASPA (AAH29128).

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Amino Acids
    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Other
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Amino acid metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa nuclear extract lysate (ab14655)
    • Raji whole cell lysate (ab30124)
    • MOLT4 whole cell lysate (ab7912)
  • Recombinant Protein

    • Recombinant Human ASPA protein (ab123155)

Applications

Our Abpromise guarantee covers the use of ab97454 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 36 kDa.
IHC-P 1/100 - 1/500.

Target

  • Function

    Catalyzes the deacetylation of N-acetylaspartic acid (NAA) to produce acetate and L-aspartate. NAA occurs in high concentration in brain and its hydrolysis NAA plays a significant part in the maintenance of intact white matter. In other tissues it act as a scavenger of NAA from body fluids.
  • Tissue specificity

    Brain white matter, skeletal muscle, kidney, adrenal glands, lung and liver.
  • Involvement in disease

    Defects in ASPA are the cause of Canavan disease (CAND) [MIM:271900]; also known as spongy degeneration of the brain. CAND is a rare neurodegenerative condition of infancy or childhood characterized by white matter vacuolization and demeylination that gives rise to a spongy appearance. The clinical features are onset in early infancy, atonia of neck muscles, hypotonia, hyperextension of legs and flexion of arms, blindness, severe mental defect, megalocephaly, and death by 18 months on the average.
  • Sequence similarities

    Belongs to the AspA/AstE family. Aspartoacylase subfamily.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Target information above from: UniProt accession P45381 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 509432 Cow
    • Entrez Gene: 443 Human
    • Entrez Gene: 11484 Mouse
    • Entrez Gene: 100142661 Pig
    • Omim: 608034 Human
    • SwissProt: P45381 Human
    • SwissProt: Q8R3P0 Mouse
    • Unigene: 171142 Human
    • Unigene: 293574 Mouse
    see all
  • Alternative names

    • ACY 2 antibody
    • ACY-2 antibody
    • ACY2 antibody
    • ACY2_HUMAN antibody
    • Aminoacylase 2 antibody
    • Aminoacylase-2 antibody
    • Aminoacylase2 antibody
    • ASP antibody
    • ASPA antibody
    • Aspartoacylase (aminoacylase 2, Canavan disease) antibody
    • Aspartoacylase (Canavan disease) antibody
    • Aspartoacylase antibody
    • NUR 7 antibody
    • NUR7 antibody
    • OTTMUSP00000006437 antibody
    • RP23-213I10.1 antibody
    • Small lethargic antibody
    see all

Images

  • Western blot - Anti-ASPA antibody (ab97454)
    Western blot - Anti-ASPA antibody (ab97454)
    All lanes : Anti-ASPA antibody (ab97454) at 1/1000 dilution

    Lane 1 : MOLT4 whole cell lysate
    Lane 2 : Raji whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 36 kDa



    10% SDS PAGE
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASPA antibody (ab97454)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASPA antibody (ab97454)
    ab97454 at 1/100 dilution staining ASPA in paraffin-embedded Cal27 xenograft by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

Protocols

  • Immunohistochemistry protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (1)

    Publishing research using ab97454? Please let us know so that we can cite the reference in this datasheet.

    ab97454 has been referenced in 1 publication.

    • Gessler DJ  et al. Redirecting N-acetylaspartate metabolism in the central nervous system normalizes myelination and rescues Canavan disease. JCI Insight 2:e90807 (2017). PubMed: 28194442

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