Recombinant
RabMAb

Recombinant Anti-ASPA antibody [EPR22072] - BSA and Azide free (ab239522)

Overview

  • Product name

    Anti-ASPA antibody [EPR22072] - BSA and Azide free
    See all ASPA primary antibodies
  • Description

    Rabbit monoclonal [EPR22072] to ASPA - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, IHC-Fr, WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human ASPA aa 50-300. The exact sequence is proprietary.
    Database link: P45381

  • Positive control

    • IHC-P: Mouse cerebrum tissue.
  • General notes

    Ab239522 is the carrier-free version of ab223269. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab239522 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab239522 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.

Perform heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

WB Use at an assay dependent concentration. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
IP Use at an assay dependent concentration.

Target

  • Function

    Catalyzes the deacetylation of N-acetylaspartic acid (NAA) to produce acetate and L-aspartate. NAA occurs in high concentration in brain and its hydrolysis NAA plays a significant part in the maintenance of intact white matter. In other tissues it act as a scavenger of NAA from body fluids.
  • Tissue specificity

    Brain white matter, skeletal muscle, kidney, adrenal glands, lung and liver.
  • Involvement in disease

    Defects in ASPA are the cause of Canavan disease (CAND) [MIM:271900]; also known as spongy degeneration of the brain. CAND is a rare neurodegenerative condition of infancy or childhood characterized by white matter vacuolization and demeylination that gives rise to a spongy appearance. The clinical features are onset in early infancy, atonia of neck muscles, hypotonia, hyperextension of legs and flexion of arms, blindness, severe mental defect, megalocephaly, and death by 18 months on the average.
  • Sequence similarities

    Belongs to the AspA/AstE family. Aspartoacylase subfamily.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • ACY 2 antibody
    • ACY-2 antibody
    • ACY2 antibody
    • ACY2_HUMAN antibody
    • Aminoacylase 2 antibody
    • Aminoacylase-2 antibody
    • Aminoacylase2 antibody
    • ASP antibody
    • ASPA antibody
    • Aspartoacylase (aminoacylase 2, Canavan disease) antibody
    • Aspartoacylase (Canavan disease) antibody
    • Aspartoacylase antibody
    • NUR 7 antibody
    • NUR7 antibody
    • OTTMUSP00000006437 antibody
    • RP23-213I10.1 antibody
    • Small lethargic antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling ASPA with ab223269 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear and cytoplasmic staining on gliocytes of mouse cerebrum (PMID: 29116375). Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab223269).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen rat cerebrum tissue labeling ASPA with ab223269 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Nuclear and cytoplasmic staining on gliocytes of rat cerebrum. (PMID: 29116375).

    The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223269).

  • ASPA was immunoprecipitated from 0.35 mg human brain lysate with ab223269 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab223269 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

    Lane 1: Human brain lysate 10 µg (Input).
    Lane 2: ab223269 IP in human brain lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab223269 in human brain lysate.

    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 10 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223269).

  • Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue labeling ASPA with ab223269 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear and cytoplasmic staining on gliocytes of rat cerebral cortex (PMID: 29116375). Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223269).

  • Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling ASPA with ab223269 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear and cytoplasmic staining on gliocytes of mouse cerebrum (PMID: 29116375). Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223269).

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling ASPA with ab223269 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear and cytoplasmic staining mainly on proximal renal tubules of human kidney (PMID: 16935940). Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223269).

  • Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling ASPA with ab223269 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear and cytoplasmic staining on gliocytes of human cerebrum (PMID: 29116375). Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223269).

  • Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebrum tissue labeling ASPA with ab223269 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Nuclear and cytoplasmic staining on gliocytes of mouse cerebrum (PMID: 29116375).

    The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223269).

References

ab239522 has not yet been referenced specifically in any publications.

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