• Product name
    Asparaginase Activity Assay Kit
  • Detection method
  • Sample type
    Cell Lysate
  • Assay type
    Enzyme activity
  • Assay time
    0h 40m
  • Product overview

    Asparaginase Activity Assay Kit (Colorimetric/Fluorometric) (ab107922) provides a simple, direct and automation-ready procedure for measuring asparaginase activity in biological samples. In the assay, Asparaginase hydrolyzes asparagine to generate aspartic acid, which can be detected indirectly colorimetrically (OD=570 nm) or fluorescently (Ex/Em = 535/590 nm) using a coupled enzymatic reaction.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Asparaginase (EC is a homotetramer that catalyzes the hydrolysis of asparagine to aspartic acid and ammonia and exhibits about a 2-4% activity on glutamine and 5% on D-asparagine. Asparaginase does not occur naturally in humans but is found in bacteria, plants and many animals (e.g. guinea pigs).
    Asparaginases can be used for different industrial and pharmaceutical purposes. The most common use is in food manufacturing as food processing aid to reduce acrylamide, a suspected carcinogen, produced in fried starchy food products. Metabolization of asparaginase prevents acrylamide formation in fried foods (Maillard reaction).
    Other asparaginases are used to treat acute lymphoblastic leukemia (ALL) and some other hematopoietic neoplasms (e.g. multiple myeloma). The enzyme’s antineoplastic effects are based on the inability of cancer cells (unlike healthy cells) to synthesize asparagine. However, the enzyme is not without some antigenicity and toxicity so it is very important to measure its activity in biological samples or monitor its activity during therapy.

  • Platform



  • Typical asparaginase standard calibration curve using colorimetric reading.

  • Typical asparaginase standard calibration curve using fluorometric reading.

  • Asparaginase measured in cell lysates showing quantity (mU) per mL of tested sample.

  • Aspartate measured in cell lysates after 10 min and 30 min incubation time showing quantity (nmol) per mL of tested sample.



This product has been referenced in:
  • Kim K  et al. Cell mass-dependent expression of an anticancer protein drug by tumor-targetedSalmonella. Oncotarget 9:8548-8559 (2018). Read more (PubMed: 29492216) »

See 1 Publication for this product

Customer reviews and Q&As

It is ideal to collect serum from fresh blood samples, aliquot them and store at -80 degrees C for short periods of time (a couple of months) for this assay. EDTA is usually avoided in enzymatic assays because of its chelating properties.
I a...

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This assay works on the principle of the Asparaginase present in your samples to hydrolyze asparagine to produce aspartic acid. This aspartic acid has to be further converted to another intermediate which finally reacts with the probe on order to gener...

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Thank you for your enquiry and interest in our products.
This assay kit (ab107922) works on the principle of the Asparaginase present in your samples to hydrolyze Asparagine to produce Aspartic acid. This Aspartic acid has to be further converted ...

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we are not so certain that whole blood can be used as a sample type with this kit.
The red colour of the blood will interfere significantly with the final reading.
We would recommend using plasma/serum samples for this purpose. Plasma/serum ca...

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