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Asparaginase Activity Assay Kit (Colorimetric/Fluorometric) (ab107922) provides a simple, direct and automation-ready procedure for measuring asparaginase activity in biological samples. In the assay, Asparaginase hydrolyzes asparagine to generate aspartic acid, which can be detected indirectly colorimetrically (OD=570 nm) or fluorescently (Ex/Em = 535/590 nm) using a coupled enzymatic reaction.
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Asparaginase (EC 22.214.171.124) is a homotetramer that catalyzes the hydrolysis of asparagine to aspartic acid and ammonia and exhibits about a 2-4% activity on glutamine and 5% on D-asparagine. Asparaginase does not occur naturally in humans but is found in bacteria, plants and many animals (e.g. guinea pigs).
Asparaginases can be used for different industrial and pharmaceutical purposes. The most common use is in food manufacturing as food processing aid to reduce acrylamide, a suspected carcinogen, produced in fried starchy food products. Metabolization of asparaginase prevents acrylamide formation in fried foods (Maillard reaction).
Other asparaginases are used to treat acute lymphoblastic leukemia (ALL) and some other hematopoietic neoplasms (e.g. multiple myeloma). The enzyme’s antineoplastic effects are based on the inability of cancer cells (unlike healthy cells) to synthesize asparagine. However, the enzyme is not without some antigenicity and toxicity so it is very important to measure its activity in biological samples or monitor its activity during therapy.
|Asparaginase Assay Buffer||WM||1 x 25ml|
|Aspartate Enzyme Mix (lyophilized)||Green||1 vial|
|Aspartate Standard (100 mM)||Yellow||1 x 0.1ml|
|Conversion Mix (lyophilized)||Purple||1 vial|
|Positive Control (lyophilized)||Blue||1 vial|
|Probe (in DMSO)||Red||1 x 0.2ml|
|Substrate Mix (lyophilized)||Orange||1 vial|
Typical asparaginase standard calibration curve using colorimetric reading.
Typical asparaginase standard calibration curve using fluorometric reading.
Asparaginase measured in cell lysates showing quantity (mU) per mL of tested sample.
Aspartate measured in cell lysates after 10 min and 30 min incubation time showing quantity (nmol) per mL of tested sample.
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