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    asparaginase-antibody-ab55824.pdf

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Signal Transduction Metabolism Amino Acids
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Anti-Asparaginase antibody (ab55824)

  • Datasheet
  • SDS
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Key features and details

  • Rabbit polyclonal to Asparaginase
  • Suitable for: WB, ELISA
  • Reacts with: Recombinant fragment
  • Isotype: IgG

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Overview

  • Product name

    Anti-Asparaginase antibody
    See all Asparaginase primary antibodies
  • Description

    Rabbit polyclonal to Asparaginase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Recombinant fragment
    Predicted to work with: Soybean
  • Immunogen

    Synthetic peptide corresponding to Soybean Asparaginase (N terminal).
    Database link: Q84L89

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol (glycerin, glycerine), PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Amino Acids
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Amino acid metabolism
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab55824 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 37 kDa. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
ELISA
Use at an assay dependent dilution.
Notes
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 37 kDa. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
ELISA
Use at an assay dependent dilution.

Target

  • Relevance

    Asparaginase is an enzyme purified from E. coli and Erwinia carotovora. It acts by deaminating extracellular L asparagine, an amino acid that appears to be essential for protein synthesis by some tumour cells which are unable to synthesise asparagine. Asparaginase from Erwinia carotovora is serologically and biochemically distinct from asparaginase from E. coli, although its antineoplastic activity and toxicity is similar. Asparaginase is usually considered to be cell cycle phase nonspecific, but it may block some cells in G1 or S phase. Asparaginase reduces cellular and humoral immunity. E.coli contains two L asparaginase isoenzymes: L asparaginase I, a low affinity enzyme located in the cytoplasm, and L asparaginase II, a high affinity secreted enzyme.
  • Cellular localization

    Cytoplasmic, Secreted and Periplasm.
  • Alternative names

    • AnsA antibody
    • AnsB antibody
    • Colaspase antibody
    • Cytoplasmic asparaginase I antibody
    • L ASNase I antibody
    • L ASNase II antibody
    • L asparaginase 1 antibody
    • L asparaginase 2 antibody
    • L asparaginase I antibody
    • L asparaginase II antibody
    • L asparaginase II precursor antibody
    • L asparagine amidohydrolase I antibody
    • L asparagine amidohydrolase II antibody
    see all

Protocols

  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (2)

Publishing research using ab55824? Please let us know so that we can cite the reference in this datasheet.

ab55824 has been referenced in 2 publications.

  • Chang JH  et al. Sleeping Beauty Transposon-Mediated Asparaginase Gene Delivery by a Nanoparticle Platform. Sci Rep 9:11457 (2019). PubMed: 31391525
  • Zenatti PP  et al. Low Bioavailability and High Immunogenicity of a New Brand of E. colil-Asparaginase with Active Host Contaminating Proteins. EBioMedicine 30:158-166 (2018). ELISA . PubMed: 29550241

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