Anti-Asparaginase antibody (ab55824)
Key features and details
- Rabbit polyclonal to Asparaginase
- Suitable for: WB, ELISA
- Reacts with: Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-Asparaginase antibody
See all Asparaginase primary antibodies -
Description
Rabbit polyclonal to Asparaginase -
Host species
Rabbit -
Tested applications
Suitable for: WB, ELISAmore details -
Species reactivity
Reacts with: Recombinant fragment
Predicted to work with: Soybean
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Immunogen
Synthetic peptide corresponding to Soybean Asparaginase (N terminal).
Database link: Q84L89 -
General notes
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One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab55824 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 37 kDa. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
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| ELISA |
Use at an assay dependent dilution.
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| Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 37 kDa. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein. |
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ELISA
Use at an assay dependent dilution. |
Target
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Relevance
Asparaginase is an enzyme purified from E. coli and Erwinia carotovora. It acts by deaminating extracellular L asparagine, an amino acid that appears to be essential for protein synthesis by some tumour cells which are unable to synthesise asparagine. Asparaginase from Erwinia carotovora is serologically and biochemically distinct from asparaginase from E. coli, although its antineoplastic activity and toxicity is similar. Asparaginase is usually considered to be cell cycle phase nonspecific, but it may block some cells in G1 or S phase. Asparaginase reduces cellular and humoral immunity. E.coli contains two L asparaginase isoenzymes: L asparaginase I, a low affinity enzyme located in the cytoplasm, and L asparaginase II, a high affinity secreted enzyme. -
Cellular localization
Cytoplasmic, Secreted and Periplasm. -
Alternative names
- AnsA antibody
- AnsB antibody
- Colaspase antibody
see all
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab55824 has been referenced in 2 publications.
- Chang JH et al. Sleeping Beauty Transposon-Mediated Asparaginase Gene Delivery by a Nanoparticle Platform. Sci Rep 9:11457 (2019). PubMed: 31391525
- Zenatti PP et al. Low Bioavailability and High Immunogenicity of a New Brand of E. colil-Asparaginase with Active Host Contaminating Proteins. EBioMedicine 30:158-166 (2018). ELISA . PubMed: 29550241