Anti-Asparaginase antibody (ab55824)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameAnti-Asparaginase antibody
See all Asparaginase primary antibodies -
DescriptionRabbit polyclonal to Asparaginase
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Host speciesRabbit
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Tested applicationsSuitable for: WB, ELISAmore details
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Species reactivityReacts with: Recombinant fragment
Predicted to work with: Soybean
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Immunogen
A synthetic peptide corresponding to N terminal residues of soybean (Glycine max) L asparaginase
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: 50% Glycerol, PBS -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab55824 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 37 kDa. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein. | |
| ELISA | Use at an assay dependent dilution. |
Target
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RelevanceAsparaginase is an enzyme purified from E. coli and Erwinia carotovora. It acts by deaminating extracellular L asparagine, an amino acid that appears to be essential for protein synthesis by some tumour cells which are unable to synthesise asparagine. Asparaginase from Erwinia carotovora is serologically and biochemically distinct from asparaginase from E. coli, although its antineoplastic activity and toxicity is similar. Asparaginase is usually considered to be cell cycle phase nonspecific, but it may block some cells in G1 or S phase. Asparaginase reduces cellular and humoral immunity. E.coli contains two L asparaginase isoenzymes: L asparaginase I, a low affinity enzyme located in the cytoplasm, and L asparaginase II, a high affinity secreted enzyme.
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Cellular localizationCytoplasmic, Secreted and Periplasm.
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Alternative names
- AnsA antibody
- AnsB antibody
- Colaspase antibody
see all
Protocols
Datasheets and documents
References
This product has been referenced in:
- Zenatti PP et al. Low Bioavailability and High Immunogenicity of a New Brand of E. colil-Asparaginase with Active Host Contaminating Proteins. EBioMedicine 30:158-166 (2018). ELISA . Read more (PubMed: 29550241) »
