Biotin Anti-Asparaginase antibody (ab34616)
Key features and details
- Biotin Rabbit polyclonal to Asparaginase
- Suitable for: WB, ELISA
- Reacts with: Escherichia coli
- Conjugation: Biotin
- Isotype: IgG
Overview
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Product name
Biotin Anti-Asparaginase antibody
See all Asparaginase primary antibodies -
Description
Biotin Rabbit polyclonal to Asparaginase -
Host species
Rabbit -
Conjugation
Biotin -
Tested applications
Suitable for: WB, ELISAmore details -
Species reactivity
Reacts with: Escherichia coli -
Immunogen
Asparaginase (Escherichia coli )
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.5
Preservative: 0.01% Sodium azide
Constituents: 1% BSA, 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading...
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Purity
IgG fraction -
Purification notes
This antibody was purified by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab34616 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 37 kDa.
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ELISA |
Use at an assay dependent concentration.
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Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 37 kDa. |
ELISA
Use at an assay dependent concentration. |
Target
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Relevance
Asparaginase is an enzyme purified from E. coli and Erwinia carotovora. It acts by deaminating extracellular L asparagine, an amino acid that appears to be essential for protein synthesis by some tumour cells which are unable to synthesise asparagine. Asparaginase from Erwinia carotovora is serologically and biochemically distinct from asparaginase from E. coli, although its antineoplastic activity and toxicity is similar. Asparaginase is usually considered to be cell cycle phase nonspecific, but it may block some cells in G1 or S phase. Asparaginase reduces cellular and humoral immunity. E.coli contains two L asparaginase isoenzymes: L asparaginase I, a low affinity enzyme located in the cytoplasm, and L asparaginase II, a high affinity secreted enzyme. -
Cellular localization
Cytoplasmic, Secreted and Periplasm. -
Database links
- Entrez Gene: 1036607 Escherichia coli
- Entrez Gene: 1039460 Escherichia coli
- Entrez Gene: 946278 Escherichia coli
- Entrez Gene: 959650 Escherichia coli
- SwissProt: P00805 Escherichia coli
- SwissProt: P0A962 Escherichia coli
- SwissProt: P0A963 Escherichia coli
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Alternative names
- AnsA antibody
- AnsB antibody
- Colaspase antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab34616 has not yet been referenced specifically in any publications.