Overview

  • Product name

    Anti-Asparagine synthetase antibody
    See all Asparagine synthetase primary antibodies
  • Description

    Rabbit polyclonal to Asparagine synthetase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to region within amino acids 1 -227 of the Human Asparagine synthetase protein (P08243).

  • Positive control

    • K562 cell lysates and Human Cal27 xenograft.

Properties

Applications

Our Abpromise guarantee covers the use of ab126254 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 64 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Pathway

    Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (L-Gln route): step 1/1.
  • Sequence similarities

    Contains 1 asparagine synthetase domain.
    Contains 1 glutamine amidotransferase type-2 domain.
  • Information by UniProt
  • Database links

  • Alternative names

    • asnS antibody
    • ASNS_HUMAN antibody
    • ASNSD antibody
    • Asparagine synthetase [glutamine-hydrolyzing] antibody
    • Asparagine synthetase antibody
    • Cell cycle control protein TS11 antibody
    • Glutamine dependent asparagine synthetase 3 antibody
    • Glutamine dependent asparagine synthetase antibody
    • Glutamine hydrolyzing antibody
    • Glutamine-dependent asparagine synthetase antibody
    • OTTHUMP00000024510 antibody
    • OTTHUMP00000204938 antibody
    • OTTHUMP00000204939 antibody
    • OTTHUMP00000204940 antibody
    • OTTHUMP00000204941 antibody
    • OTTHUMP00000204942 antibody
    • TS11 antibody
    • TS11 cell cycle control protein antibody
    see all

Images

  • Anti-Asparagine synthetase antibody (ab126254) at 1/1000 dilution + K562 whole cell lysate at 30 µg

    Predicted band size: 64 kDa



    Gel concentration: 7.5%
  • ab126254, at 1/500, staining Asparagine synthetase in Human Cal27 xenograftby immunohistochemistry [Paraffin Embedded Tissues (IHC-P).

References

This product has been referenced in:

  • Du F  et al. SOX12 promotes colorectal cancer cell proliferation and metastasis by regulating asparagine synthesis. Cell Death Dis 10:239 (2019). Read more (PubMed: 30858360) »
See 1 Publication for this product

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