Overview

  • Product name

    Anti-Asparagine synthetase antibody
    See all Asparagine synthetase primary antibodies
  • Description

    Rabbit polyclonal to Asparagine synthetase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Asparagine synthetase aa 511-561. The exact sequence is proprietary.
    Database link: P08243

  • Positive control

    • IP: HEK-293T whole cell lysate.
  • General notes

    ab226413 has not performed satisfactorily when used for WB of Asparagine Synthetase in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of Asparagine Synthetase.

Properties

Applications

Our Abpromise guarantee covers the use of ab226413 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Pathway

      Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (L-Gln route): step 1/1.
    • Sequence similarities

      Contains 1 asparagine synthetase domain.
      Contains 1 glutamine amidotransferase type-2 domain.
    • Information by UniProt
    • Database links

    • Alternative names

      • asnS antibody
      • ASNS_HUMAN antibody
      • ASNSD antibody
      • Asparagine synthetase [glutamine-hydrolyzing] antibody
      • Asparagine synthetase antibody
      • Cell cycle control protein TS11 antibody
      • Glutamine dependent asparagine synthetase 3 antibody
      • Glutamine dependent asparagine synthetase antibody
      • Glutamine hydrolyzing antibody
      • Glutamine-dependent asparagine synthetase antibody
      • OTTHUMP00000024510 antibody
      • OTTHUMP00000204938 antibody
      • OTTHUMP00000204939 antibody
      • OTTHUMP00000204940 antibody
      • OTTHUMP00000204941 antibody
      • OTTHUMP00000204942 antibody
      • TS11 antibody
      • TS11 cell cycle control protein antibody
      see all

    Images

    • Asparagine synthetase was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 20% of IP loaded) with ab226413 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab226413 at 0.4 µg/ml.

      Lane 1: ab226413 IP in HEK-293T whole cell lysate.

      Lane 2: Control IgG IP in HEK-293T whole cell lysate.

      Detection: Chemiluminescence with exposure time of 10 seconds.

    References

    ab226413 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab226413.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up