Recombinant Anti-Asparagine synthetase antibody [EP282Y] (ab40850)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP282Y] to Asparagine synthetase
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-Asparagine synthetase antibody [EP282Y]
See all Asparagine synthetase primary antibodies -
Description
Rabbit monoclonal [EP282Y] to Asparagine synthetase -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human Asparagine synthetase aa 500-600 (C terminal). The exact sequence is proprietary.
-
Positive control
- K562 cell lysate, human breast carcinoma tissue and human muscle tissue.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP282Y -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab40850 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
1/1000 - 1/2000. Detects a band of approximately 60 kDa (predicted molecular weight: 61 kDa).
|
Notes |
---|
WB
1/1000 - 1/2000. Detects a band of approximately 60 kDa (predicted molecular weight: 61 kDa). |
Target
-
Pathway
Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (L-Gln route): step 1/1. -
Sequence similarities
Contains 1 asparagine synthetase domain.
Contains 1 glutamine amidotransferase type-2 domain. - Information by UniProt
-
Database links
- Entrez Gene: 440 Human
- Entrez Gene: 27053 Mouse
- Entrez Gene: 25612 Rat
- Omim: 108370 Human
- SwissProt: P08243 Human
- SwissProt: Q61024 Mouse
- SwissProt: P49088 Rat
- Unigene: 489207 Human
see all -
Alternative names
- asnS antibody
- ASNS_HUMAN antibody
- ASNSD antibody
see all
Images
-
Anti-Asparagine synthetase antibody [EP282Y] (ab40850) at 1/2000 dilution + K562 cell lysate at 10 µg
Predicted band size: 61 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (6)
ab40850 has been referenced in 6 publications.
- Nishikawa G et al. Targeting Asparagine Synthetase in Tumorgenicity Using Patient-Derived Tumor-Initiating Cells. Cells 11:N/A (2022). PubMed: 36291140
- Mukherjee A et al. Asparagine Synthetase Is Highly Expressed at Baseline in the Pancreas Through Heightened PERK Signaling. Cell Mol Gastroenterol Hepatol 9:1-13 (2020). PubMed: 31421261
- Restall IJ et al. Brain Tumor Stem Cell Dependence on Glutaminase Reveals a Metabolic Vulnerability through the Amino Acid Deprivation Response Pathway. Cancer Res 80:5478-5490 (2020). PubMed: 33106333
- Huang ML et al. Molecular and functional alterations in a mouse cardiac model of Friedreich ataxia: activation of the integrated stress response, eIF2a phosphorylation, and the induction of downstream targets. Am J Pathol 183:745-57 (2013). WB ; Mouse . PubMed: 23886890
- Scotti C et al. Cell-cycle inhibition by Helicobacter pylori L-asparaginase. PLoS One 5:e13892 (2010). WB, IHC-P ; Human . PubMed: 21085483
- Pos Z et al. Histamine suppresses fibulin-5 and insulin-like growth factor-II receptor expression in melanoma. Cancer Res 68:1997-2005 (2008). PubMed: 18339882