Recombinant
RabMAb

Recombinant Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] (Alexa Fluor® 568) (ab221286)

Overview

  • Product name

    Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] (Alexa Fluor® 568)
  • Description

    Rabbit monoclonal [EPR12145] to Aspartate Aminotransferase + FABP-1 (Alexa Fluor® 568)
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 568. Ex: 578nm, Em: 603nm
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human Aspartate Aminotransferase aa 150-250 (Cysteine residue). The exact sequence is proprietary.
    Database link: P17174

  • Positive control

    • ICC/IF: MCF7 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab221286 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50.

This product gave a positive signal in MCF7 cells fixed with 100% methanol (5 min)

Target

Images

  • ab221286 staining Aspartate Aminotransferase + FABP-1 in MCF7 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab221286 at 1/50 dilution (pseudocolored in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

References

ab221286 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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