Recombinant
RabMAb

Recombinant Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] - BSA and Azide free (ab232471)

Overview

  • Product name

    Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] - BSA and Azide free
  • Description

    Rabbit monoclonal [EPR12145] to Aspartate Aminotransferase + FABP-1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Aspartate Aminotransferase aa 150-250 (Cysteine residue). The exact sequence is proprietary.
    Database link: P17174

  • Positive control

    • IHC-P: Human glioma tissue.
  • General notes

    Ab232471 is the carrier-free version of ab170950. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab232471 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab232471 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

We do not guarantee IHC-P for mouse and rat.

WB Use at an assay dependent concentration. Predicted molecular weight: 46 kDa.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

Target

Images

  • Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling Aspartate Aminotransferase + FABP-1 using unpurified ab170950 at 1/50 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170950).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] (ab170950) at 1/1000 dilution + Recombinant human FABP-1 protein (ab206788) at 0.015 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 46 kDa
    Observed band size: 47 kDa
    why is the actual band size different from the predicted?


    Exposure time: 180 seconds


    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170950).

  • Flow Cytometry analysis of K-562 (Human chronic myelogenous leukemia lymphoblast) cells labeling Aspartate Aminotransferase + FABP-1 with purified ab170950 at 1:20 dilution (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor ® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170950).

  • Immunocytochemistry/Immunofluorescence analysis of HT-29 (human colorectal adenocarcinoma) cells labelling Aspartate Aminotransferase + FABP-1 with purified ab170950 at 1/120. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (ab150077) at 1/1000 dilution was used as the secondary antibody. Nuclei counterstained with DAPI (blue). 

    Secondary Only Control: PBS was used instead of the primary antibody as the negative control. 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170950).

  • Immunofluorescent analysis of HepG2 cells labeling Aspartate Aminotransferase + FABP-1 using unpurified ab170950 at 1/50 dilution (green). DAPI nuclear staining (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170950).

  • Flow cytometric analysis of permeabilized K562 cells labeling Aspartate Aminotransferase + FABP-1 using unpurified ab170950 at 1/10 dilution (red)  or a rabbit IgG negative (green).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170950).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human glioma tissue sections labeling Aspartate Aminotransferase + FABP-1 with Purified ab170950 at 1:170 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170950).

References

ab232471 has not yet been referenced specifically in any publications.

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