Recombinant
RabMAb

Recombinant Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] (HRP) (ab201765)

Overview

  • Product name

    Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] (HRP)
  • Description

    Rabbit monoclonal [EPR12145] to Aspartate Aminotransferase + FABP-1 (HRP)
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human Aspartate Aminotransferase aa 150-250 (Cysteine residue). The exact sequence is proprietary.
    Database link: P17174

  • Positive control

    • WB: HepG2; MCF-7 and K562 whole cell lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab201765 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).

Target

Images

  • All lanes : Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] (HRP) (ab201765) at 1/5000 dilution

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 3 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 46 kDa
    Observed band size: 46 kDa


    Exposure time: 4 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab201765 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

References

ab201765 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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