Product nameAnti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] (HRP)
DescriptionRabbit monoclonal [EPR12145] to Aspartate Aminotransferase + FABP-1 (HRP)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide within Human Aspartate Aminotransferase aa 150-250 (Cysteine residue). The exact sequence is proprietary.
Database link: P17174
- WB: HepG2; MCF-7 and K562 whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
Storage bufferpH: 7.4
Preservative: 0.1% Proclin
Constituents: PBS, 30% Glycerol, 1% BSA
Concentration information loading...
PurityProtein A purified
- Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] (ab170950)
- Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] (Alexa Fluor® 647) (ab201043)
- Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] (Alexa Fluor® 568) (ab221286)
- Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] - BSA and Azide free (ab232471)
Our Abpromise guarantee covers the use of ab201765 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).|
Cellular localizationAspartate Aminotransferase: Cytoplasm. FABP-1: Mitochondrion matrix. Cell membrane. Exposure to alcohol promotes translocation to the cell membrane.
All lanes : Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] (HRP) (ab201765) at 1/5000 dilution
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 3 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 46 kDa
Observed band size: 46 kDa
Exposure time: 4 minutes
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab201765 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
ab201765 has not yet been referenced specifically in any publications.